Optimization of Insulin Rapid Amyloid Fibrosis Conditions

• Published in: 东华大学学报（英文版） Vol. 40; no. 4; pp. 370 - 376
• Main Authors: HUANG Chen, LIU Yaojia, LI Yimeng, ZHANG Kai, CHEN Xiang, MA Shutao, LI Zhen, CHEN Ting
• Format: Journal Article
• Language: English
• Published: College of Biological Science and Medical Engineering,Donghua University,Shanghai 201620,China 31.08.2023
• Subjects: insulin; amyloid fibrosis; model protein; response surface analysis
• ISSN: 1672-5220
• DOI: 10.19884/j.1672-5220.202212006

Q71; Amyloid fibers are now considered as one of the possible therapeutic targets of neurodegenerative diseases due to their unique physicochemical properties and toxicity.To efficiently trace in real time how the drug inhibits protein amyloid fibrosis,it is necessary to study the conditions for rapid amyloid fibrosis.Insulin is selected as the model protein in vitro to explore the process of amyloid formation.The effects of the molar concentration of NaCl,pH and reaction temperature in the single-factor experiment are discussed and the response surface analysis is carried out.Amyloid fibrosis is labeled by Thioflavin-T(ThT).The optimal molar concentration of NaCl,pH and reaction temperature for insulin rapid amyloid fibrosis are 50.0 mmol/L,2.02 and 54℃,respectively.With the addition of 0.1 mmol/L phenol,the half-time of insulin amyloid fibrosis is shortened from 5.4 h to 1.7 h.The insulin rapid amyloid fibrosis system provides a new approach for screening the protein amyloid fibrosis inhibitors.