Stenotrophomonas terrae应用于退化窖泥修复的初步研究
TS261.1; 为修复退化窖泥,该研究将Stenotrophomonas terrae接种于退化窖泥,以正常窖泥及退化窖泥为对照,将不同窖泥进行模拟发酵实验,对发酵结束后的窖泥、糟醅、酒样理化指标及细菌菌群进行检测,并对不同窖泥样品细菌菌群进行主坐标分析(PCoA)及相关性网络分析.结果表明,与退化窖泥相比,修复后的窖泥pH、产酸能力、铵态氮含量、感官评分分别显著提升至4.82、185.83 mg/g、98.33 mg/100 g、6.10分(P＜0.05);对应糟醅的酸度显著降低至3.27(P＜0.05),而水分及残余淀粉含量差异不显著(P＞0.05);对应酒样总酯、己酸乙酯含量显著提升至...
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| Published in | 中国酿造 Vol. 43; no. 9; pp. 209 - 214 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
刘念酿酒师技能大师工作室,四川成都 611130
30.10.2024
四川省食品发酵工业研究设计院有限公司,四川成都 611130 酿酒生物技术及应用四川省重点实验室,四川宜宾 644000 中国轻工业酿酒工程及应用重点实验室,四川成都 611130%四川全兴酒业有限公司,四川成都 611637 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0254-5071 |
| DOI | 10.11882/j.issn.0254-5071.2024.09.033 |
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| Abstract | TS261.1; 为修复退化窖泥,该研究将Stenotrophomonas terrae接种于退化窖泥,以正常窖泥及退化窖泥为对照,将不同窖泥进行模拟发酵实验,对发酵结束后的窖泥、糟醅、酒样理化指标及细菌菌群进行检测,并对不同窖泥样品细菌菌群进行主坐标分析(PCoA)及相关性网络分析.结果表明,与退化窖泥相比,修复后的窖泥pH、产酸能力、铵态氮含量、感官评分分别显著提升至4.82、185.83 mg/g、98.33 mg/100 g、6.10分(P＜0.05);对应糟醅的酸度显著降低至3.27(P＜0.05),而水分及残余淀粉含量差异不显著(P＞0.05);对应酒样总酯、己酸乙酯含量显著提升至1.00 g/L、0.56 g/L(P＜0.05),总酸由0.49 g/L显著降低至0.27 g/L(P＜0.05).修复窖泥细菌菌群丰富度及多样性增加,基于属水平,乳杆菌属(Lactobacillus)相对丰度极显著降低(P＜0.01),氢孢菌属(Hydrogenispora)、Clostridium_sensu_stricto_12、嗜碱菌属(Alkalibaculum)及产己酸菌属(Caproiciproducens)等功能微生物相对丰度极显著提高(P＜0.01).PCoA及相关性网络分析表明,退化窖泥接种Stenotrophomonas terrae后,可使其细菌菌群接近于正常窖泥,可增强窖泥细菌间的协同作用,提高微生物生态网络稳定性. |
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| AbstractList | TS261.1; 为修复退化窖泥,该研究将Stenotrophomonas terrae接种于退化窖泥,以正常窖泥及退化窖泥为对照,将不同窖泥进行模拟发酵实验,对发酵结束后的窖泥、糟醅、酒样理化指标及细菌菌群进行检测,并对不同窖泥样品细菌菌群进行主坐标分析(PCoA)及相关性网络分析.结果表明,与退化窖泥相比,修复后的窖泥pH、产酸能力、铵态氮含量、感官评分分别显著提升至4.82、185.83 mg/g、98.33 mg/100 g、6.10分(P＜0.05);对应糟醅的酸度显著降低至3.27(P＜0.05),而水分及残余淀粉含量差异不显著(P＞0.05);对应酒样总酯、己酸乙酯含量显著提升至1.00 g/L、0.56 g/L(P＜0.05),总酸由0.49 g/L显著降低至0.27 g/L(P＜0.05).修复窖泥细菌菌群丰富度及多样性增加,基于属水平,乳杆菌属(Lactobacillus)相对丰度极显著降低(P＜0.01),氢孢菌属(Hydrogenispora)、Clostridium_sensu_stricto_12、嗜碱菌属(Alkalibaculum)及产己酸菌属(Caproiciproducens)等功能微生物相对丰度极显著提高(P＜0.01).PCoA及相关性网络分析表明,退化窖泥接种Stenotrophomonas terrae后,可使其细菌菌群接近于正常窖泥,可增强窖泥细菌间的协同作用,提高微生物生态网络稳定性. |
| Abstract_FL | In order to repair degraded pit mud,the Stenotrophomonas terrae was inoculated to the degraded pit mud,with normal pit mud and degraded pit mud as the control,the physicochemical indexes and bacterial flora of pit mud,fermented grains and Baijiu samples after fermentation were de-tected,and principal coordinate analysis(PCoA)and correlation network analysis were carried out for bacterial flora of different pit mud samples.The results showed that the pH,acid-producing capacity,ammonium nitrogen content and sensory scores of the repaired pit mud were significantly increased to 4.82,185.83 mg/g,98.33 mg/100 g,and 6.10(P＜0.05).The acidity of the corresponding fermented grains was significantly decreased to 3.27(P＜0.05).There were no significant differences in moisture and residual starch contents(P＞0.05).The contents of total esters and ethyl caproate in corresponding Baijiu samples were significantly improved to 1.00 g/L and 0.56 g/L(P＜0.05),and the total acid was significantly decreased from 0.49 g/L to 0.27 g/L(P＜0.05).The bacterial flora richness and diversity of repaired mud increased.Based on genus level,the relative abundance of Lacto-bacillus decreased significantly(P＜0.01).The relative abundance of functional microorganisms of Hydrogenispora,Clostridium_sensu_stricto_12,Alkalibaculum and Caproiciproducens significantly increased(P＜0.01).PCoA and correlation network analysis showed that after inoculation with S.terrae,the microbial flora could be close to that of normal pit mud,which could enhance the synergistic effect among bacterial in pit mud and im-prove the stability of microbial ecological network. |
| Author | 常少健 张磊 刘义会 杨朝彬 李觅 朱建行 邱显平 陈心雨 蔡海燕 |
| AuthorAffiliation | 四川省食品发酵工业研究设计院有限公司,四川成都 611130;酿酒生物技术及应用四川省重点实验室,四川宜宾 644000;刘念酿酒师技能大师工作室,四川成都 611130;中国轻工业酿酒工程及应用重点实验室,四川成都 611130%四川全兴酒业有限公司,四川成都 611637 |
| AuthorAffiliation_xml | – name: 四川省食品发酵工业研究设计院有限公司,四川成都 611130;酿酒生物技术及应用四川省重点实验室,四川宜宾 644000;刘念酿酒师技能大师工作室,四川成都 611130;中国轻工业酿酒工程及应用重点实验室,四川成都 611130%四川全兴酒业有限公司,四川成都 611637 |
| Author_FL | CAI Haiyan CHANG Shaojian CHEN Xinyu ZHU Jianhang ZHANG Lei QIU Xianping YANG Chaobin LIU Yihui LI Mi |
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| Keywords | 窖泥 bacterial diversity remediation 理化指标 Stenotrophomonas terrae 修复 细菌多样性 pit mud physicochemical indexes |
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| Publisher | 刘念酿酒师技能大师工作室,四川成都 611130 四川省食品发酵工业研究设计院有限公司,四川成都 611130 酿酒生物技术及应用四川省重点实验室,四川宜宾 644000 中国轻工业酿酒工程及应用重点实验室,四川成都 611130%四川全兴酒业有限公司,四川成都 611637 |
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| Title | Stenotrophomonas terrae应用于退化窖泥修复的初步研究 |
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