Enzymatic formation of consecutive thymine-Hg-thymine base pairs by DNA polymerases

From the perspective of the preparation of a DNA-based nanowire containing an array of metal ions, DNA-polymerase-catalyzed primer extension reactions were investigated and the formation of up to ten consecutive T-Hg II -T base pairs was achieved by the Hg II -mediated primer extension reaction in t...

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Published inChemical communications (Cambridge, England) Vol. 56; no. 8; pp. 1225 - 1228
Main Authors Funai, Tatsuya, Tagawa, Chizuko, Nakagawa, Osamu, Wada, Shun-ichi, Ono, Akira, Urata, Hidehito
Format Journal Article
LanguageEnglish
Published 14.10.2020
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Summary:From the perspective of the preparation of a DNA-based nanowire containing an array of metal ions, DNA-polymerase-catalyzed primer extension reactions were investigated and the formation of up to ten consecutive T-Hg II -T base pairs was achieved by the Hg II -mediated primer extension reaction in the presence of Mn II ions. This enzymatic approach may be one of the promising techniques for preparing a DNA-based metal array. Ten consecutive T-Hg II -T base pairs were successfully formed by DNA polymerase-catalyzed primer extension reactions.
Bibliography:10.1039/d0cc04423g
Electronic supplementary information (ESI) available: Experimental procedures and additional figures and tables. See DOI
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc04423g