A fluorescent "ON-OFF-ON" switch for the selective and sequential detection of Hg and I with applications in imaging using human AGS gastric cancer cells
A new fluorescent "on-off-on" probe (BIPQ) is designed and developed which selectively binds with Hg 2+ ; its emission intensity is quenched almost 40-fold at 455 nm without interference from other metal cations. On gradual addition of I − to the solution of BIPQ-Hg 2+ , the emission rever...
Saved in:
Published in | Dalton transactions : an international journal of inorganic chemistry Vol. 49; no. 1; pp. 187 - 195 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
17.12.2019
|
Online Access | Get full text |
Cover
Loading…
Summary: | A new fluorescent "on-off-on" probe (BIPQ) is designed and developed which selectively binds with Hg
2+
; its emission intensity is quenched almost 40-fold at 455 nm without interference from other metal cations. On gradual addition of I
−
to the solution of BIPQ-Hg
2+
, the emission reverts to its original intensity. The limits of detection of BIPQ for Hg
2+
and I
−
are found to be on the order of 3.12 × 10
−9
and 5.48 × 10
−8
M, respectively, which shows clearly that BIPQ can sense Hg
2+
at a very minute level. DFT and TDDFT studies are conducted with the probe to establish similarity between theoretical and experimental outcomes. Finally, to demonstrate its practical benefit in biological fields, live cell imaging experiments with BIPQ are carried out to detect Hg
2+
in human AGS gastric cancer cell lines.
A new fluorescent "on-off-on" probe (BIPQ) probe was designed which displayed selective and sequential detection of Hg
2+
and I
−
without interference of other cations or anions. |
---|---|
Bibliography: | CCDC Electronic supplementary information (ESI) available: NMR and MS of all new compounds, limit of detection determination, quantum yield calculation For ESI and crystallographic data in CIF or other electronic format see DOI 10.1039/C9DT04245H 1914359 etc |
ISSN: | 1477-9226 1477-9234 |
DOI: | 10.1039/c9dt04245h |