Development of antibody conjugates for pre-targeting radioimmunotherapy to describe tumor penetration of therapeutic molecules

• Published in: Annals of biomedical engineering Vol. 28; no. SUPPL. 1; pp. S - 21
• Main Authors: Hamblett, Kevin, Meyer, Damon, Hamlin, Don, Corcoran, Melissa, Press, Oliver, Wilbur, Scott, Stayton, Patrick
• Format: Journal Article
• Language: English
• Published: 01.01.2000
• Subjects: Bioassay; Cells; Controlled drug delivery; Hemodynamics; Immunology; Radiotherapy; Tumors

To determine the effect of streptavidin-biotin affinity on tumor penetration, streptavidin mutants spanning a wide range of affinities and off-rates for biotin were conjugated to the antibody 1F5. Flow cytometry and radioimmunoassays were used to verify the binding of single chain antibody scFv) to...