Development of antibody conjugates for pre-targeting radioimmunotherapy to describe tumor penetration of therapeutic molecules

To determine the effect of streptavidin-biotin affinity on tumor penetration, streptavidin mutants spanning a wide range of affinities and off-rates for biotin were conjugated to the antibody 1F5. Flow cytometry and radioimmunoassays were used to verify the binding of single chain antibody scFv) to...

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Bibliographic Details
Published inAnnals of biomedical engineering Vol. 28; no. SUPPL. 1; pp. S - 21
Main Authors Hamblett, Kevin, Meyer, Damon, Hamlin, Don, Corcoran, Melissa, Press, Oliver, Wilbur, Scott, Stayton, Patrick
Format Journal Article
LanguageEnglish
Published 01.01.2000
Subjects
Bioassay
Cells
Controlled drug delivery
Hemodynamics
Immunology
Radiotherapy
Tumors
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Summary:To determine the effect of streptavidin-biotin affinity on tumor penetration, streptavidin mutants spanning a wide range of affinities and off-rates for biotin were conjugated to the antibody 1F5. Flow cytometry and radioimmunoassays were used to verify the binding of single chain antibody scFv) to Blymphoma cells.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-2
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SourceType-Conference Papers & Proceedings-1
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ISSN:0090-6964