In Vivo and Ex Vivo View of Newt Lens Regeneration

Lens regeneration in the adult newt illustrates a unique example of naturally occurring cell transdifferentiation. During this process, iris pigmented epithelial cells (iPECs) reprogram into a lens, a tissue that is derived from a different embryonic source. Several methodologies both in vivo and in...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2562; p. 197
Main Authors Tsissios, Georgios, Sallese, Anthony, Chen, Weihao, Miller, Alyssa, Wang, Hui, Del Rio-Tsonis, Katia
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
Animals
Cell Transdifferentiation
Epithelial Cells
Lens, Crystalline
Salamandridae
Explant
Optical Coherence Tomography
Reprogramming
Transdifferentiation
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Summary:Lens regeneration in the adult newt illustrates a unique example of naturally occurring cell transdifferentiation. During this process, iris pigmented epithelial cells (iPECs) reprogram into a lens, a tissue that is derived from a different embryonic source. Several methodologies both in vivo and in culture have been utilized over the years to observe this phenomenon. Most recently, Optical Coherence Tomography (OCT) has been identified as an effective tool to study the lens regeneration process in continuity through noninvasive, real-time imaging of the same animal. Described in this chapter are three different methodologies that can be used to observe the newt lens regeneration process both in vivo and ex vivo.
ISSN:1940-6029
DOI:10.1007/978-1-0716-2659-7_13