Human Corneal Rim Perfusion Culture

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2858; p. 113
• Main Author: Mao, Weiming
• Format: Journal Article
• Language: English
• Published: United States 2025
• Subjects: Cell Culture Techniques - instrumentation; Cell Culture Techniques - methods; Cornea - cytology; Humans; Organ Culture Techniques - instrumentation; Organ Culture Techniques - methods; Perfusion - instrumentation; Perfusion - methods; Printing, Three-Dimensional; Trabecular Meshwork - cytology; Trabecular Meshwork - metabolism; Corneal rim perfusion culture; Outflow facility; Intraocular pressure
• ISSN: 1940-6029
• DOI: 10.1007/978-1-0716-4140-8_10

Human anterior segment perfusion cultures are frequently used for trabecular meshwork research. However, this model requires the use of whole eye globes which are expensive. Here, we describe a method using human corneal rims as an alternative to anterior segments for perfusion culture. In this method, the human corneal rim is glued to a three-dimensional-printed perfusion plates instead of using mechanical compression in traditional perfusion culture setup. The corneal rim and perfusion plate are placed onto a special holder for perfusion culture. This method is affordable and easy to set up. The instruments for traditional perfusion culture studies can be used for this model without modification.