miR-181a-5p regulates cell proliferation and apoptosis by targeting Kras in the breast cancer cell line MDA-MB-231

• Published in: Jie fang jun yi xue za zhi Vol. 43; no. 9; p. 735
• Main Authors: Jian-miao HE, Hua-zhou, ZHAO, WANG, Ting, Xiao-chen, QIU, Jian-feng, WENG, Xin-hui, ZHANG, Zhi-yu, CAO
• Format: Journal Article
• Language: Chinese
• Published: Beijing People's Military Medical Press 01.09.2018
• Subjects: Apoptosis; Breast cancer; Cell cycle; Cell growth
• ISSN: 0577-7402
• DOI: 10.11855/j.issn.0577-7402.2018.09.03

Objective 　To explore the potential function of miR-181a-5p in regulating cell proliferation and apoptosis in the breast cancer cell line MDA-MB-231 by targeting Kras. Methods 　The expressions of miR-181a-5p and Kras in MDA-MB-231 (a human breast cancer cell line) and HBL-100 (a human breast cell line) were detected by RT-PCR and Western blotting, respectively. The MDA-MB-231 cells were transfected with miR-181a-5p mimics. Cells that were transfected with the negative control mimics were served as control. To verify the upregulation of miR-181a-5p using its mimics, the expression of miR-181a-5p was detected using RT-PCR and the expression of its target, Kras, was detected using Western blotting. Cell proliferation was measured by MTT assay. Cell apoptosis and cell cycle were detected with flow cytometer. To confirm that Kras is a real target of miR-181a-5p in MDA-MB-231 cells, wild-type Kras 3'-UTR plasmid (Kras-wt) or mutant Kras 3'-UTR plasmid (Kras-Mut) were co-transfected with miR-181a-5p mimic or control