Expression of the regulatory B cells in peripheral blood of patients with lung cancer and its clinical significance

• Published in: Jie fang jun yi xue za zhi Vol. 40; no. 5; p. 396
• Main Authors: Bao-guo, CHEN, Li-ping, MEI, ZHENG, Rui, Bo-li, LI
• Format: Journal Article
• Language: Chinese
• Published: Beijing People's Military Medical Press 01.01.2015
• Subjects: Clinical significance; Lung cancer
• ISSN: 0577-7402
• DOI: 10.11855/j.issn.0577-7402.2015.05.13

Objective　To investigate the expression and clinical significance of regulatory B cells (Bregs) in peripheral blood of patients with lung cancer. Methods　The peripheral blood was collected from 72 patients with lung cancer and 29 healthy subjects (as control), and the Bregs subsets (CD19+CD5+CD1d+) were detected by flow cytometry, the levels of IL-10 and TGF-β in serum were determined by ELISA. Proportion of Bregs and peripheral blood levels of IL-10 and TGF-β were compared between lung cancer patients and healthy controls, and between the lung cancer patients in stage Ⅰ+Ⅱ and those in stage Ⅲ+Ⅳ. The correlation between Bregs proportion and peripheral blood levels of IL-10 and TGF-β were further investigated. Results　The percentage of Bregs was significantly higher in the peripheral blood of lung cancer patients than that of healthy controls (5.01%±1.20% vs 2.78%±0.56%, P<0.01). The proportion of Bregs was found to be increased in patients in clinical Ⅲ and Ⅳ stages (5.63%±1.04%) compared with those in clinic