Sox9 is indispensable for testis differentiation in the red-eared slider turtle, a reptile with temperaturedependent sex determination

Herein, Sox9 knockdown in the red-eared slider turtle (Trachemys scripta) embryos at a male-producing temperature led to complete male-to-female sex reversal, characterized by the formation of an ovary-like structure, disappearance of male marker AMH, and ectopic expression of ovarian regulator FOXL...

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Published inDōngwùxué yánjiū Vol. 42; no. 6; pp. 721 - 725
Main Authors Hui, Hang-Bo, Xiao, Ling, Sun, Wei, Zhou, Ying-Jie, Zhang, Hai-Yan, Ge, Chu-Tian
Format Journal Article
LanguageEnglish
Published Kunming Kunming Institute of Zoology, The Chinese Academy of Sciences 01.11.2021
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Summary:Herein, Sox9 knockdown in the red-eared slider turtle (Trachemys scripta) embryos at a male-producing temperature led to complete male-to-female sex reversal, characterized by the formation of an ovary-like structure, disappearance of male marker AMH, and ectopic expression of ovarian regulator FOXL2, as well as a female distribution pattern of germ cells. Immunofluorescence analysis of the control group showed that the SOX9 protein was mainly located in the nucleus of Sertoli precursor cells in MPT embryonic gonads, while no SOX9 signal was detected in the FPT embryonic gonads at stage 25 (Figure 1A). In XY mice, the deletion or a 77% reduction of Sox9 leads to complete maleto-female sex reversal, while 50% reduction causes partial sex reversal (Bagheri-Fam et al., 2020; Gonen et al., 2017; Lavery et al., 2011). [...]ectopic expression of Sox9 in XX gonads causes male development in the absence of Sry (Huang et al., 1999; Vidal et al., 2001). Unlike mammals, Sox9 expression in chickens (Gallus gallus domesticus) and American alligators (Alligator mississippiensis) occurs later than that of Amh, indicating that Amh expression is not regulated by SOX9, and Sox9 may be located downstream of the sex differentiation pathway in nonmammalian vertebrates (Hirst et al., 2018; Smith & Sinclair, 2004; Western et al., 1999).
ISSN:0254-5853
DOI:10.24272/j.issn.2095-8137.2021.136