Protective effect of SP600125 against liver cell injury in rats under repeated and sustained high +Gz exposure

• Published in: Jie fang jun yi xue za zhi Vol. 39; no. 11; p. 854
• Main Authors: Wen-bing, LI, Hong-yi, ZHANG, Ya-lin, KONG, Di-jian, MA, SHI, Bin, CHANG, Peng, HU, Shen, LIU, Lei
• Format: Journal Article
• Language: Chinese
• Published: Beijing People's Military Medical Press 01.01.2014
• Subjects: Liver; Rodents
• ISSN: 0577-7402
• DOI: 10.11855/j.issn.2577-7402.2014.11.02

Objective　To explore the effect of JNK inhibitor SP600125 on expression of JNK/c-jun in liver cells of rats under repeated and sustained high +Gz exposure and its mechanism of the effect. Methods　Eighteen inbred adult male Wistar rats were randomly divided into control group, +10Gz group and SP600125 group (n=6). The rats in +10Gz group and SP600125 group were fixed to the rotating arm of a centrifuge with head towards the axis. The increase rate of acceleration was 1G/s with a peak-time of 3 minutes, and the +Gz exposure was repeated 5 times with an interval of 30 minutes. SP600125 was given to rats of SP600125 group 30 minutes before the first centrifugation by intraperitoneal injection. All of the animals were sacrificed 30 minutes after centrifugation. Blood samples were collected from inferior vena cava to determine the plasma level of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The expression of c-jun mRNA was determined by quantitative real-time RT-PCR (qRTPCR). The expressions