Effects of percutaneous midband pulse current stimulation in hepatic region on the activity of hepatic mitochondrial Na+-K+-ATPase and Ca2+-Mg2+-ATPase in exercise-induced fatigued rats

• Published in: Jie fang jun yi xue za zhi Vol. 40; no. 4; p. 327
• Main Authors: Yi-zong ZHAI, Chang-lin, HUANG, CHANG, Qi, WANG, Jiu-qing, ZHANG, Jia, Yan-ling, GUO
• Format: Journal Article
• Language: Chinese
• Published: Beijing People's Military Medical Press 01.01.2015
• Subjects: Enzymes; Fatigue; Rodents
• ISSN: 0577-7402
• DOI: 10.11855/j.issn.0577-7402.2015.04.15

Objective　To explore the effects of percutaneous impulsive current stimulation in hepatic region on the activity of hepatic mitochondrial Na+-K+-ATPase and Ca2+-Mg2+-ATPase in exercise-induced fatigued rats, in order to investigate the effect of exercise-induced fatigue. Methods　Seventy-two 8-week old male Wistar rats were randomly divided into 4 groups (18 each): control group (group A), fatigue group (group B), stimulation before fatigue group (group C) and stimulation after fatigue group (group D). Exhaustion of animals in B, C and D groups were reproduced by prolonged swimming. Current stimulation (1024Hz, 10mA, current cycle 1sec) for 20 minutes was given to the rats of group C before swimming, and to those in group D after exhaustion. At the weekend of 1st, 3rd and 5th week after modeling, the rats were sacrificed in batches from each group (6 each). The activities of hepatic mitochondrial Na+-K+-ATPase and Ca2+-Mg2+-ATPase were determined by spectrophotometry, and Bradfood protein quantification was em