Investigation of Antagonistic Properties of Bacteria Bacillus Subtilis against Carrot Phytopathogenes in vitro and in vivo Experiments

• Published in: Journal of pharmaceutical sciences and research Vol. 10; no. 6; pp. 1619 - 1622
• Main Authors: Pershakova, T V, Kupin, G A, Mihaylyuta, L V, Babakina, M V, Panasenko, E Y, Viktorova, E P
• Format: Journal Article
• Language: English
• Published: Cuddalore Journal of Pharmaceutical Sciences and Research 01.06.2018
• Subjects: Bacteria; Carrots; Crop diseases; Crops; Harvest; Limited liability companies; Microorganisms; Pathogens; Physiology; Seeds; Tomatoes; Vegetables; Russia
• ISSN: 0975-1459

The antifungal activity of the strain of Bacillus subtilis V26 persisted under the influence of temperature, under UV treatment, also the strain was resistant to proteases. The containers were stored at temperatures of +2 °C and +25 °C. The number of microorganism cells in the snips on root crops was examined every 24 hours during 7 days at +25 °C and every 48 hours during 14 days at +2 °C. To study the effect of Bacillus subtilis on the incidence and diameter of the lesion of diseases caused by Alternaria radicina and Erwinia carotovora depending on temperature, there were made punctures with a sterile needle on the carrot roots, and a suspension of Bacillus subtilis was added at a dose of 10 pl in an amount of 1 x 107 CFU/ml. After storage at + 25 °C for 7 days, the average diameter of the lesion in reference (untreated Bacillus subtilis) samples infected with Alternaria radicina was 17.2 mm. In samples treated with Bacillus subtilis and infected with Alternaria radicina, the average diameter of the lesion was 10.5 mm. After storage at +2 °C for 7 days, the average diameter of the lesion in samples infected with Alternaria radicina was 7.7 mm. In samples treated with Bacillus subtilis and infected with Alternaria radicina, the average diameter of the lesion was 5.1 mm. After storage at +2 °C for 14 days, the average diameter of the lesion in samples infected with Alternaria radicina was 5.5 mm. In samples treated with Bacillus subtilis and infected with Alternaria radicina, the average diameter of the lesion was 7.6 mm. After storage at + 2 °C for 28 days, the average diameter of the lesion in samples infected with Alternaria radicina was 18.7 mm. In samples treated with Bacillus subtilis and infected with Alternaria radicina, the average diameter of the lesion was 12.1 mm. The effect of Bacillus subtilis on the degree of damage to wet bacterial rot caused by Erwinia carotovora in carrot roots, depending on the temperature after 7, 14 and 28 days of storage is shown in Figure 4. After storage at +25 °C for 7 days, the average diameter of the lesion in samples infected with Erwinia carotovora was 7.2 mm. In samples treated with Bacillus subtilis and infected with Erwinia carotovora, the average diameter of the lesion was 6.5 mm. After storage at +2 °C for 7 days, the average diameter of the lesion in samples infected with Erwinia carotovora was 4.2 mm. In samples treated with Bacillus subtilis and infected with Erwinia carotovora, the average diameter of the lesion was 2.2 mm. After storage at +2 °C for 14 days, the average diameter of the affected samples infected with Erwinia carotovora was 8.2 mm. In samples treated with Bacillus subtilis and infected with Erwinia carotovora, the average diameter of the lesion was 5.3 mm. After storage at +2 °C for 28 days, the average diameter of the affected samples infected with Erwinia carotovora was 12.4 mm. In samples treated with Bacillus subtilis and infected with Erwinia carotovora, the average diameter of the lesion was 9.3 mm. Conclusions On the basis of the conducted researches antagonistic properties of strains of bacteria Bacillus subtilis concerning phytopathogens of carrot Sclerotinia sclerotiorum, Alternaria radicina and Erwinia carotovora in experiments in vitro are established.