STAINING OF ECONOMICALLY IMPORTANT PARASITIC NEMATODES BY DEVELOPED DERIVATIVES OF BENZANTHRONE LUMINOPHORE

• Published in: International Multidisciplinary Scientific GeoConference : SGEM Vol. 18; no. 6.2; pp. 581 - 587
• Main Authors: Gavarane, Inese, Mezaraupe, Ligita, Rubenina, Ilze, Kirjusina, Muza, Kirilova, Elena
• Format: Conference Proceeding
• Language: English
• Published: Sofia Surveying Geology & Mining Ecology Management (SGEM) 01.01.2018
• Subjects: Animals; Body organs; Chromatography; Dyes; Ethanol; Fluorescence; Fluorescence microscopy; Health hazards; Hydrocarbons; Larvae; Lasers; Microscopy; Morphology; Muscles; Nematodes; NMR; Nuclear magnetic resonance; Organs; Parasites; Parasitic diseases; Parasitology; Pathogens; Public health; Solvents; Staining; Studies; Trichinella; Trichinellosis; Zoonoses; United Kingdom > UK; United States > US; Germany
• ISSN: 1314-2704
• DOI: 10.5593/sgem2018/6.2/S25.077

Nematodes belonging to the genus Trichinella are zoonotic parasites with a high impact on the animal trade and human health. Foodborne pathogens of Trichinella genus are known as causative agents of human trichinellosis, a disease that is a public health hazard. According to the Commission Regulation (EU) No. 2015/1375 all animals, which are potential carriers of Trichinella spp. larvae and are for human consumption, must be tested according to one of the approved tests. For assessment of developed benzanthrone luminophore P13 the Trichinella britovi larvae samples were selected. Confocal laser scanning fluorescence microscopy was applied to visualize stained larvae. Developed technique is appropriate and demonstrated sufficient results for samples preserved in 96% ethanol for up to three years long period and for larvae obtained from two years frozen muscle samples. Staining of internal organs by developed technique may be an invaluable source of data for studies of parasite structure and variety.