A Study on the Expression of CCL5,CXCR4 and Angiogenic Factors by Prostate Cancer Stem Cells

Angiogenesis could be inhibited by inhibiting CCR5 expressing vasculature, this was proposed as a novel therapy for blocking tumor metastasis.7 CCL5 exerts pro-angiogenic effects by promoting endothelial cell migration, spreading, neovessel formation, and vascular endothelial growth factor (VEGF) se...

Full description

Saved in:
Bibliographic Details
Published inAnnals of the Romanian society for cell biology Vol. 25; no. 4; pp. 1020 - 1028
Main Authors Rambatla, Pavan Kumar, Pandrangi, Shanti Latha, Rentala, Satyanarayana, Garimella, Sireesha V
Format Journal Article
LanguageEnglish
Published Arad "Vasile Goldis" Western University Arad, Romania 01.01.2021
Subjects
1-Phosphatidylinositol 3-kinase
Actin
AKT protein
Angiogenesis
Antibodies
Bone marrow
Cancer therapies
CCR5 protein
Cell culture
Cell migration
Cellular biology
Chemokines
CXCR4 protein
Endothelial cells
Hypoxia
Kinases
Medical prognosis
Metastases
Permeability
Prostate cancer
Protein-tyrosine kinase
Signal transduction
Src protein
Statistical analysis
Stem cells
Tumor cells
Tumors
Vascular endothelial growth factor
Vascularization
Online AccessGet full text

Cover

More Information
Summary:Angiogenesis could be inhibited by inhibiting CCR5 expressing vasculature, this was proposed as a novel therapy for blocking tumor metastasis.7 CCL5 exerts pro-angiogenic effects by promoting endothelial cell migration, spreading, neovessel formation, and vascular endothelial growth factor (VEGF) secretion. [...]tumor cells, upon CCL5 stimulation, canproduce VEGF or, by secreting CCL5, may recruit CCR5-expressing TAMs. Inhibition of CCL5/CCR5 axis can lead to the development of new therapeutic strategies for treatment of cancer.89 CCL5 was shown to promote VEGF-dependent angiogenesis through the PI3K/Akt signaling pathway and the downregulation of miR-200b.10 CCL5 neutralization was shown to inhibit tumor growth and it rendered the tumors more sensitive to PDGFR inhibitor treatment.11 Neovascularization is essential for tumor survival and growth. The protein tyrosine kinase c-Src was shown to mediate the expression of VEGF and promote the angiogenic potential of cancer cells20. c-Src was shown to be necessary for the vascular permeability activity of VEGF12 (Eliceri et al. 1999). The blots were stripped and reprobed for anti-ßactin to determine equivalent loading. ß-actin was also used as the control housekeeping protein. 6) Statistical Analysis:Data were analyzed by one-way ANOVA followedby Dunnett's t-test using GraphPad InStat version 3.05
ISSN:2067-3019
2067-8282