ALP (Alkaline Phosphatase) Expression in Simple Fracture Incident in Rat (Rattus Norvegicus) Femur Bone Supplemented by Apis Mellifera Honey

The aim of the experiment was to observe the serum alkaline phosphatase (ALP) expression of Apis mellifera honey supplementation on simple fracture femur bone. Twelve adult male rats were divided into 4 groups. Those groups are control group, which supplemented with aquadest for 1,5 ml and 3 groups...

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Published inJournal of International Dental & Medical Research Vol. 13; no. 3; pp. 887 - 891
Main Authors Hasib, Abdullah, Wahjuningrum, Dian Agustin, Ibrahim, Muhammad Huda Ramadhan, Kurniawan, Hendy Jaya, Ernawati, Rizky, Hadinoto, Maria Elisea Kiswantoro, Mooduto, Latief
Format Journal Article
LanguageEnglish
Published Diyarbakir Ectodermal Dysplasia Group - Turkey 01.09.2020
Subjects
Alkaline phosphatase
Bees
Bioactive compounds
Bone marrow
Bones
Drug dosages
Estrogens
Experiments
Femur
Flavonoids
Fractures
Honey
Laboratories
Mineralization
Organic matter
Osteotomy
Phosphatase
Surgery
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Summary:The aim of the experiment was to observe the serum alkaline phosphatase (ALP) expression of Apis mellifera honey supplementation on simple fracture femur bone. Twelve adult male rats were divided into 4 groups. Those groups are control group, which supplemented with aquadest for 1,5 ml and 3 groups were supplemented with 1,5 ml honey solution (1g/Kg BW, 2g/Kg BW, 3g/Kg BW) for 2 weeks. Fracture incident was made at the day before supplementation by osteotomy technique using dental bur in the metaphyseal region of femur reaching inside the bone marrow. At the fifteenth day, serum was obtained by centrifuging the blood than assessed using prestige analyzer 24i based on IFCC method. The data were analyzed using ANOVA and Tukey with SPSS for windows. The result showed a very significantly different (P<0. 01) between honey groups and control group and there was no significant different (P>0. 05) between honey groups. The indication of raising ALP revealed that honey might impact osteoblast. The result indicates that gradual dosage on simple fracture might not affect seriously as honey was organic matter containing bioactive compound as minor constituent not in the form of highly intensive extract. Thus, Apis mellifera honey might enhance ALP serum concentration in the incident of simple fracture.Experimental article (J Int Dent Med Res 2020; 13(3): 887-891) I
ISSN:1309-100X
1309-100X