Collection of Metal ions and Separation of Mercury(II) Ion with Dithiocarbamic Acid Derivative of Sephadex

• Published in: Nippon Kagakukai shi (1972) Vol. 1984; no. 8; p. 1273
• Main Authors: HAYASHI, Shigehiko, AOYAMA, Kinuyo, YOSHIMURA, Kazuko, SHIODA, Toshiyuki, DEGUCHI, Koichi, KOTSUJI, Keiya
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Science and Technology Agency 01.08.1984
• ISSN: 0369-4577
• DOI: 10.1246/nikkashi.1984.1273

Carboxymethyl Sephadex C-25 (medium) was converted to hydrazinocarbonylmethyl Sephadex(HD-Sephadex) by the reaction with hydrazine hydrate-ethanol solution. N'-(Dithiocarboxy)hydrazinocarbonylmethyl Sephadex (DH-Sephadex) was prepared by the reaction of HD-Sephadex with carbon disulfide in aqueous sodium hydroxide solution. Mercury (II), copper (II), lead (II), cadmium (II) zinc (II), nickel (II), cobalt (II) and manganese (II) were collected completely from 50 ml of 1 x 10-5 mol[sm middot]l-1 solution of each metal salt over the pH range of 1.0-10.3, 3.5-8.4, 4.6-5.0, 4.7-8.2, 5.0-6.7, 5.1-8.6, 5.2-8.3 and 5.6-9.6, respectively, by stirring with 0.1g of DH-Sephadex for 30 min at room temperature. The separation of mercury from manganese was carried out as follows. Both 5.00 x 10-4 mmol of two metals were collected together by passing 500 ml of a mixed metal solution at pH to 6.8 through a column packed with 0.2 g of DH-Sephadex. Manganese was eluted with 1 x 10-1 mol[sm middot]l-1 EDTA solution and then mercury left on DHSephadex was eluted with conc. hydrochloric acid. The separation of mercury from other metals such as zinc and lead was also successful by the similar procedure. The separation of mercury from cobalt was carried out as follows. Mercury was collected by passing 500 ml sample solution containing 5.00 x 10-4 mmol each of these metals at pH 1.1 through a column packed with 0.1 g of DH-Sephadex. Then cobalt in the effluent was collected with an another fresh DH-Sephadex after readjusting pH to 6.6. Mercury was eluted with conc. hydrochloric acid and DH-Sephadex collecting cobalt was dissolved with a mixture of 30%hydrogen peroxide solution(0.5 ml) and 18 mol[sm middot]l-1 sulfuric acid(2ml) at about 90°C in the column. Similarly, quantitative separation of mercury from other metals such as manganese, nickel, zinc, cadmium and lead was also achieved.