Antimicrobial and cytotoxic activities screening of fungal secondary metabolites isolated from marine sponge Callyspongia sp

Secondary metabolites from marine sponge-derived fungi repeatedly showed potential bioactive activity against several diseases. In this study, screening for antibacterial and cytotoxic isolated fungi from marine sponge Callyspongia sp. have been performed. A common media for culturing fungi, sodium...

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Published inAquaculture, Aquarium, Conservation & Legislation Vol. 14; no. 1; pp. 249 - 258
Main Authors Handayani, Dian, Artasasta, Muh A, Mutia, Dian, Atikah, Nurul, Rustini, Tallei, Trina E
Format Journal Article
LanguageEnglish
Published Cluj-Napoca Bioflux SRL 01.02.2021
Subjects
Acetates
Acetic acid
Antibiotics
Antimicrobial activity
Antimicrobial agents
Aquatic crustaceans
Breast cancer
Callyspongia
Color
Cotton
Cultivation
Culture media
Cytotoxicity
Dextrose
Disease resistance
Drug resistance
E coli
Ethyl acetate
Fungi
Grain cultivation
Identification
Lethality
Marine invertebrates
Metabolites
Mortality causes
Pathogens
Phytochemicals
Reagents
Room temperature
Screening
Secondary metabolites
Shellfish
Sodium
Staphylococcus infections
Toxicity tests
Indonesia
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Summary:Secondary metabolites from marine sponge-derived fungi repeatedly showed potential bioactive activity against several diseases. In this study, screening for antibacterial and cytotoxic isolated fungi from marine sponge Callyspongia sp. have been performed. A common media for culturing fungi, sodium dextrose agar (SDA), was used. For fungal isolates the streak plate method was used. Each pure fungal isolate was cultivated on rice media under room temperature for 4-8 weeks then extracted with ethyl acetate. Thirteen pure fungal isolates were successfully obtained from Callyspongia sp. Ethyl acetate extract of all isolated fungi was tested for antimicrobial activity against pathogenic microbes using the disk diffusion method and cytotoxic activity using brine shrimp lethality test (BSLT). Of thirteen fungal isolates, only Cas 02 showed antimicrobial activity against Staphylococcus aureus (SA) ATCC 2592 and Escherichia coli (EC) ATCC 25922 with a diameter of inhibition zone of 12.75 mm and 17.16 mm respectively. The cytotoxic activity results showed that only isolate Cas03 had a potential activity with LC50 < 30 µg mL-1 and four fungal strains (Cas02, Cas06, Cas07, and Cas09) had a moderate activity with LC50 < 100 µg mL-1. Molecular identification showed that Cas02 was Aspergillus unguis, Cas03 was Penicillium citrinum, Cas06 and Cas07 were A. flavus, and Cas09 was A. austroafricanus.
ISSN:1844-8143
1844-9166