Mass Chromatography of Esters Composed of Saturated Higher Normal Fatty Acids and Normal Fatty Alcohols

• Published in: Nippon Kagakukai shi (1972) Vol. 1976; no. 12; p. 1862
• Main Authors: OHTA, Tadao, NAMBA, Ryujiro, MATSUMOTO, Isao
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Science and Technology Agency 01.12.1976
• ISSN: 0369-4577
• DOI: 10.1246/nikkashi.1976.1862

In order to establish a quantitative state analysis of saturated wax-esters, a fundamental study of mass chromatography was carried out to the standard esters (C24∼C36) which were composed of saturated higher normal fatty acids (C12∼C18) and normal fatty alcohols (C12∼C18). The following three equations were found to hold among the relative fragment ion intensities, 011+ of the constituent acidOH 'acid from estersper molecule, that of the constituent alcohol (C, F12, +) /alcohol, and their carbon number (n, m). (1) if n is constant, acid=al (n+m) +b1 (Fig.4) (2) if n+ m is constant, /, d=a2n+b2 (Fig.3) (3) if m is constant, /alcohol=as (n In) b3 (Fig.4) where, al, a3 and 122 are positive constant values a2, 121 and b3 are negative constant values Equations (1) and (2) indicate the possibility of a rapid quantitative state micro analysis of wax-esters having the same carbon number, but different combinations of acid and alcohol. This technique was applied to the analysis of spermaceti and new informations were obtained. The time required for the analysis was approximately 30 minutes and coefficient of variation for the result was 1.12%.