DNA barcoding of Aphanius vladykovi from different habitats in Chaharmahal va Bakhtiari Province, Iran

• Published in: International journal of aquatic biology Vol. 8; no. 1; pp. 66 - 72
• Main Authors: Koupaie, Farideh Abolhasani, Segherloo, Iraj Hashemzadeh, Heydarnejad, Mohamad Saieed, Tabatabaei, Seyedeh Narjes
• Format: Journal Article
• Language: English
• Published: Karaj Iranian Society of Ichthyology 01.02.2020
• Subjects: Aphanius vladykovi; Conservation; Cytochrome; Cytochrome oxidase I; Cytochrome-c oxidase; Cytochromes; Deoxyribonucleic acid; Distance; DNA; DNA barcoding; Gene sequencing; Genetic distance; Habitats; Haplotypes; Internet; Mitochondria; Oxidase; Phylogeny; Population genetics; Population studies; Populations; Rivers; Trees; Type localities; Wetlands; Iran
• ISSN: 2383-0956; 2322-5270
• DOI: 10.22034/ijab.v8i1.612

This study was aimed to reveal the possible cryptic diversity of the Aphanius viaclykovi populations io the Chaharmahal va Bakhtiari Provioce, fran usiog mitochondrial cytochrome-c oxidase subuoit I (COl). A total of 30 specimeos from the Beheshtabad River, Choghakhor and Gandoman Wetlands, and Brovi, Shalamzar, and Balagholi springs from the Chaharmahal va Bakhtiari Province were collected. The maximum within-population genetic distance based on K2P was 0.28% and this distance was 0.22% between populations of Gandoman and Brovi with Shalmazar, whereas the least genetic distance was observed between Choghakhor and Beheshtabad (0.09%). A total of six haplotypes were observed between the studied specimens. Maximum Likelihood (ML) and Neighbor-Joining (NJ) trees reconstructed and all haplotypes from A. via clyko vi specimens collected from non-type localities nested in one group with A. viaclykovi from Choghakhor wetland i.e. type locality. The results of this study detected no cryptic diversity in A. vladykoviinhabiting different habitats in the studied region. Hence, it is proposed to consider all the studied populations in conservation measures related to A. viaclycovi. Athcle history: Received 4 June 2019 Accepted 6 January 2020 Available online 25 February 2020 Keywords: Cytochrome oxidase I Differentiation Genetic barcodes Flaplotypes Phylogeny Abstract: This study was aimed to reveal the possible cryptic diversity of the Aphanius vladykovi populations in the Chaharmahal va Bakhtiari Province, Iran using mitochondrial cytochrome-c oxidase subunit I (COI). A total of 30 specimens from the Beheshtabad River, Choghakhor and Gandoman Wetlands, and Brovi, Shalamzar, and Balagholi springs from the Chaharmahal va Article history: Received 4 June 2019 Accepted 6 January 2020 Available online 25 February 2020 Bakhtiari Province were collected. The maximum within-population genetic distance based on K2P was 0.28% and this distance was 0.22% between populations of Gandoman and Brovi with Shalmazar, whereas the least genetic distance was observed between Choghakhor and Beheshtabad (0.09%). A total of six haplotypes were observed between the studied specimens. Maximum Likelihood (ML) and Neighbor-Joining (NJ) trees reconstructed and all haplotypes from A. vladykovi specimens collected from non-type localities nested in one group with A. vladykovi from Choghakhor wetland i.e. type locality. The results of this study detected no cryptic diversity in A. vladykovi inhabiting different habitats in the studied region. Hence, it is proposed to consider all the studied populations in conservation measures related to A. vladycovi.