Local synbiotic from Amorphophallus muelleri Bl. and Bacillus sp. to boost Litopenaeus vannamei non-specific immune responses

Disease management is still an obstacle in Litopennaeus vannamei culture. For example, in 2009, a new disease appeared in brackish water shrimp species, namely EMS (Early Mortality Syndrome) caused by Vibrio parahaemolyticus with a unique strain (Vp-AHPND) plasmid (pAP1) 70 kbp. The syndrome could d...

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Published inAquaculture, Aquarium, Conservation & Legislation Vol. 15; no. 1; pp. 489 - 501
Main Authors Wananda, Alif Z J, Setyawan, Yhogie A, Setiani, Fia S, Wulandari, Indi E, Andriawan, Soni
Format Journal Article
LanguageEnglish
Published Cluj-Napoca Bioflux SRL 28.02.2022
Subjects
Amorphophallus
Antibiotics
Bacillus
Bacteria
Brackish water
Cell culture
Cells
Diet
Disease
Fisheries
Hemocytes
Immune response
Immune system
Immunity
Laboratories
Leukocytes (granulocytic)
Litopenaeus vannamei
Mortality
Phagocytosis
Phosphatase
Plasmids
Prebiotics
Probiotics
Production increases
Shellfish
Statistical analysis
Statistical methods
Survival
Vibriosis
Indonesia
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Summary:Disease management is still an obstacle in Litopennaeus vannamei culture. For example, in 2009, a new disease appeared in brackish water shrimp species, namely EMS (Early Mortality Syndrome) caused by Vibrio parahaemolyticus with a unique strain (Vp-AHPND) plasmid (pAP1) 70 kbp. The syndrome could decrease the survival rate of L. vannamei dramatically after infection. This study aimed to examine the effect of synbiotic (Amorphophallus muelleri Bl. and Bacillus sp.) mixed in the commercial feed to boost L. vannamei immune system against V. parahaemolyticus. The study was conducted with 4 treatments, including T0 (commercial antibiotic), T1 (3% prebiotics), T2 (2% probiotics and 3% prebiotics), T3 (4% probiotic and 3% prebiotic), T4 (6% probiotic and 3% prebiotic). Furthermore, all data was analyzed using statistical analysis that is provided by SPPS program. The results showed that the administration of synbiotics consisting of 2% probiotic and 3% prebiotic increased most parameters, including total hemocyte count (THC), granulocytes cells, total weight, and survival rate reaching 13.4 x 106 cells mL-1, 58.67 %, 0.182 g, and 50 %. Moreover, the highest number of phagocytosis was generated by T1, accounting for 65.67 %, while four treatments (T0, T1, T3, and T4) had a similar level for hyaline. Based on the results, the present study suggests that 2% probiotic and 3% prebiotic (T2) could be applied in the L. vannamei culture as a synbiotic to improve the immune system and growth performance.
ISSN:1844-8143
1844-9166