First detection of Megalocytivirus in oysters (Crassostrea iredalei) from Marudu Bay, Sabah, Malaysia

Oyster cultivation in Malaysia especially in Sabah and Johor has been actively conducted due to its economical values. However, in 2012, occurrences of mortalities due to pathogens in the Marudu Bay farming site had caused large impact on the oysters (Crassostrea iredalei Faustino, 1932) production....

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Published inAquaculture, Aquarium, Conservation & Legislation Vol. 11; no. 5; pp. 1537 - 1547
Main Authors Bobby, George, Hong, Tee K, Addis, Siti N Khadijah, Wahid, Mohd Effendy A, Sung, Yeong Y, Zainathan, Sandra C
Format Journal Article
LanguageEnglish
Published Cluj-Napoca Bioflux SRL 30.10.2018
Subjects
Aquaculture
Archives & records
Capsid protein
Crassostrea
Cultivation
Deoxyribonucleic acid
Detection
DNA
Epidemics
Farms
Fish
Fisheries
Genomes
Genotypes
Hepatitis
Herpes viruses
Infections
Marine molluscs
Microbiological strains
Mollusks
Nucleotide sequence
Oysters
Pathogens
PCR
Phylogenetics
Phylogeny
Polymerase chain reaction
Proteins
R&D
Research & development
Spleen
Virology
Viruses
Southeast Asia
Malaysia
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Summary:Oyster cultivation in Malaysia especially in Sabah and Johor has been actively conducted due to its economical values. However, in 2012, occurrences of mortalities due to pathogens in the Marudu Bay farming site had caused large impact on the oysters (Crassostrea iredalei Faustino, 1932) production. This study revealed the presence/absence of Megalocytivirus in 84 samples of oysters (C. iredalei) from Marudu Bay, Sabah and Masai, Johor using Polymerase Chain Reaction (PCR) with primer that selectively amplified Major Capsid Protein (MCP) gene of Megalocytivirus. The PCR analysis showed that the occurrence of Megalocytivirus in Sabah was 5% and 50% on 2 January 2014 and 8 May 2014 sampling batches, respectively. In Johor, the occurrence was 0% for both samplings on 25 November 2013 and 8 July 2014. Sequencing and phylogenetic analyses of the samples from Sabah (2SL13 and 2SL18) suggested that the strains of the virus shared 99% and 90% similarities respectively with Megalocytivirus Sabah and Red Tux Swordtail Iridovirus and belong to the Megalocytivirus genus (Genotype 1). Thus, this study demonstrated the first detection of Megalocytivirus in a new organism, oyster (C. iredalei).
ISSN:1844-8143
1844-9166