Interleukin 2−330 single nucleotide polymorphism association with type 1 diabetes in Iraqi patients

• Published in: AIP conference proceedings Vol. 2398; no. 1
• Main Authors: Al-Husseiny, Istabraq A., Al-Malkey, Maysaa K., Hassan, Ibtesam B., Rabaan, Ali A., Kadhim, Samer S., Khlaf, Alaudeen S.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Melville American Institute of Physics 25.10.2022
• Subjects: Autoimmune diseases; Diabetes; Interleukins; Nucleotides; Polymerase chain reaction; Polymorphism; Risk analysis; Iraq
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0093594

Type 1 diabetes (T1D) is an autoimmune disease with chronic nature resulting from a combination of both factors genetic and environmental. The genetic contributors of T1D among Iraqis are unexplored enough. The study aimed to shed a light on the contribution between genetic variation of interleukin2 (IL2) gene to T1D as a risk influencer in a sample of Iraqi patients. The association between IL2−330 polymorphism (rs2069762) was investigated in 322 Iraqis (78 T1D patients and 244 volunteers as controls). Genotyping for the haplotypes using polymerase chain reaction test – specific sequence primer (PCR-SSP) for (GG, GT, and TT) genotypes corresponding to (G and T) alleles were performed. A significant association revealed a decreased frequency between the observed and expected TT genotype between patients compared to control subjects (10.26% vs 25.89%; P= 0.004); however, in term of alleles, the T allele was non significantly decreased (24.36% vs. 53.13%; P = 1.000) and the related PF values were (0.33 and 0.28 respectively). The current study demonstrated that IL2−330 SNP; TT genotype / T allele is associated with a risk of developing T1D in this sample of the Iraqi population, and that IL2−330 genetic variation confers a risk factor for T1D pathology.