Anticoagulant activity of low-molecular-weight sulfated derivatives of galactomannan from Cyamopsis tetragonoloba (L.) seeds

• Published in: Applied biochemistry and microbiology Vol. 44; no. 1; p. 98
• Main Authors: Mestechkina, N M, Shcherbukhin, V D, Bannikova, G E, Varlamov, V P, Drozd, N N, Tolstenkov, A S, Makarov, V A, Tikhonov, V E
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.01.2008
• Subjects: Anticoagulants; Enzymes; Microbiology; Molecular weight; Seeds; Sulfates
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1007/s10438-008-1017-z

Galactomannan from seeds of Cyamopsis tetragonoloba (L.) Taub. (guar) was depolymerized using immobilized enzymatic preparation celloviridin. A set of fragments whose molecular weights varied from 12.6 to 245.6 kDa was obtained. Sulfated derivatives of components of all fractions were synthesized, in which the content of HSO 3 - -groups was 48.05 ± 2.31%. All preparations exhibited anticoagulant activity, which was recorded in vitro in two tests--aIIa and aXa. The antithrombin activity (aIIa) was high (up to 65-87 U/mg) and did not depend on the molecular weight of a sulfated derivative; in the second test (aXa), the effect of molecular weight was observed. Biospecific electrophoresis allowed us to detect the ability of galactomannan sulfates to form complexes with protamine sulfate, a classic antidote to heparin. [PUBLICATION ABSTRACT]