SENSITIVITY OF AN IMMUNOENZYMATIC TEST FOR THE DETECTION OF ANTI-L. braziliensis ANTIBODIES COMPARED TO OTHER TESTS USED FOR THE DIAGNOSIS OF AMERICAN CUTANEOUS LEISHMANIASIS

• Published in: Revista do Instituto de Medicina Tropical de São Paulo Vol. 48; no. 4; p. 215
• Main Authors: Maíra Peres Ferreira, Ferreira Roselino, Ana Maria, Margarida Maria Passeri Nascimento, Juliana Machado Aires, José Fernando de Castro Figueiredo
• Format: Journal Article
• Language: English
• Published: São Paulo Instituto de Medicina Tropical de Sao Paulo 01.07.2006
• ISSN: 0036-4665; 1678-9946
• DOI: 10.1590/s0036-46652006000400008

The diagnosis of American cutaneous leishmaniasis (ACL) is frequently based on clinical and epidemiological data associated with the results of laboratory tests. Some laboratory methods are currently being applied for the diagnosis of ACL, among them the indirect immunofluorescence reaction (IIFR), the Montenegro skin test (MST), histopathological examination, and the polymerase chain reaction (PCR). The performance of these methods varies in a considerable proportion of patients. After the standardization of an immunoenzymatic test (ELISA) for the detection of IgG in the serum of patients with ACL using a crude Leishmania braziliensis antigen, the results obtained were compared to those of other tests routinely used for the diagnosis. The tests revealed the following sensitivity, when analyzed separately: 85% for ELISA IgG, 81% for PCR, 64.4% for MST, 58.1% for IIFR, and 34% for the presence of parasites in the biopsy. ELISA was positive in 75% of patients with ACL presenting a negative MST, in 84.8% of ACL patients with negative skin or mucous biopsies for the presence of the parasite, and in 100% of cases with a negative PCR. Thus, ELISA presented a higher sensitivity than the other tests and was useful as a complementary method for the diagnosis of ACL.