Effects and mechanisms of fibronectin on adhesion and migration of different types of lung cancer cell lines

• Published in: Zhongguo fei ai za zhi Vol. 7; no. 2
• Main Authors: SONG, Liqiang, LI, Yan, NI, Diantao, QI, Haowen
• Format: Journal Article
• Language: Chinese
• Published: Tianjin Chinese Anti-Cancer Association Chinese Antituberculosis Association 01.04.2004
• Subjects: Lung cancer
• ISSN: 1009-3419; 1999-6187
• DOI: 10.3779/cjlc.v7i2.2529

目的 观察和探讨纤维连接蛋白(FN)对不同类型肺癌细胞侵袭能力的影响及机制.方法 在FN包被的培养板和FN滤膜的Boyden 浸润小室等体外侵袭模型中,比较小细胞肺癌细胞系054A和肺腺癌细胞系A549粘附及迁移能力的差别,并检测FN对肿瘤细胞增殖活性的影响.分别给予细胞抗α3整合素、抗α5整合素、抗β1整合素单抗预处理后,观察侵袭性的变化.结果 FN增强A549细胞粘附、迁移及增殖活性的作用明显大于054A细胞.这种作用在A549细胞能被抗α3、抗α5和抗β1单抗抑制,而在054A细胞能被抗α3和抗β1单抗抑制.结论 FN对不同类型肺癌细胞侵袭能力的影响不同,可能与细胞膜整合素受体的差异有关. Objective To observe and explore the effects and mechanisms of fibronectin (FN) on invasion of different types of lung cancers. Methods Using tumor invasion models in vitro of plates coated with FN and Boyden chambers with FN filter, differences of adhesion and migration between small cell lung cancer cell line (054A) and adenocarcinoma cell line (A549) were investigated, and proliferative effects of FN on cells were examined. In the meantime the invasive capability changes were observed after cell suspensions were preincubated with anti-α 5, anti-α 3 and anti-β 1 integrin antibodies, respectively. Results FN could improve the adhesion, migration and proliferation of A549 more markedly than that of 054A. The numb