Effects of Porphyromonas gingivalis lipopolysaccharide on apoptotic genes in foam cells

• Published in: Zhonghua kouqiang yixue zazhi Vol. 45; no. 5; p. 274
• Main Authors: Li, Hou-xuan, Yan, Fu-hua, Lei, Lang
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2010
• Subjects: Apoptosis - drug effects; Caspase 3 - metabolism; Cell Line, Tumor; Cell Proliferation - drug effects; Foam Cells - cytology; Foam Cells - drug effects; Foam Cells - metabolism; Gene Expression; Humans; Lipopolysaccharides - isolation & purification; Lipopolysaccharides - pharmacology; Lipoproteins, LDL - pharmacology; Macrophages - physiology; Minor Histocompatibility Antigens; Porphyromonas gingivalis - chemistry; Proto-Oncogene Proteins c-bcl-2 - metabolism; Proto-Oncogene Proteins c-myc - metabolism; Tumor Suppressor Protein p53 - metabolism
• ISSN: 1002-0098

To investigate the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on apoptotic genes in foam cells. Macrophages from THP-1 monocytes and foam cells from macrophages by oxLDL inducement were treated with oxidized low density lipoprotein (oxLDL) or oxLDL+ Pg-LPS. Cell apoptosis was detected by acridine orange-ethidium bromide (AO-EB) staining. Eleven atherosclerotic related apoptotic genes were examined with polymerase chain reaction (PCR) array, and apoptotic gene p53, c-Myc and caspase-3 were evaluated with real-time PCR. Pg-LPS enhanced cell apoptosis rate during and after foam cells formation [(5.47+/-0.93)% vs. (7.50+/-0.54)%]. PCR array demonstrated that it increased B-cell CLL-lymphoma 2 (BCL2) related protein A1 (BCL2A1) transcription during foam cells formation (>2 fold), and promoted BCL2 and BCL2A1 transcription after foam cells formation (>2 fold). It promoted p53 and caspase-3 transcription level (4.50x10(-3)+/-4.02x10(-4) vs. 5.30x10(-2)+/-4.58x10(-3)), whereas inhibited c-Myc tra