Feasibility of monitoring clopidogrel resistance with flow cytometric analysis of platelet vasodilator stimulated phosphoprotein phosphorylation

To evaluate the feasibility of monitoring clopidogrel resistance with flow cytometric analysis of platelet vasodilator stimulated phosphoprotein (VASP) phosphorylation. Twenty patients with coronary heart disease (CHD) and 17 healthy volunteers were examined for platelet aggregation rate and phospho...

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Published inNan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 34; no. 3; p. 434
Main Authors Li, Guanghua, Luo, Yanfei, Luo, Ying, Lin, Ting, Fan, Xiaobin, Liang, Ling, Xie, Hongdong, Huang, Jingwei
Format Journal Article
LanguageChinese
Published China 01.03.2014
Subjects
Aged
Aged, 80 and over
Case-Control Studies
Cell Adhesion Molecules - chemistry
Drug Resistance
Female
Flow Cytometry
Humans
Male
Microfilament Proteins - chemistry
Middle Aged
Phosphoproteins - chemistry
Phosphorylation
Platelet Function Tests
Ticlopidine - analogs & derivatives
Ticlopidine - pharmacology
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Summary:To evaluate the feasibility of monitoring clopidogrel resistance with flow cytometric analysis of platelet vasodilator stimulated phosphoprotein (VASP) phosphorylation. Twenty patients with coronary heart disease (CHD) and 17 healthy volunteers were examined for platelet aggregation rate and phosphorylation of VASP (calculated as platelet reactivity index, PRI) using traditional optical nephelometry and flow cytometry before and after concurrent therapy of clopidogrel and aspirin. No significant differences were found in PRI between CHD group and healthy control group [(89.45∓5.22)% vs (86.58∓4.35)%] before treatment. The PRI in CHD group was significantly lowered after treatment to (67.66∓19.77)% (P<0.05). Clopidogrel resistance was found in 6 (30%) cases in CHD group by flow cytometric analysis, which showed a higher sensitivity than optical nephelometry (10%). Flow cytometric analysis of VASP phosphorylation is a more reliable test to specifically evaluate clopidogrel resistance.
ISSN:1673-4254