Impact of NF-kappaB inhibitor on STAT3 translocation in PC-3 prostate cancer cell line

• Published in: Zhonghua nan ke xue Vol. 19; no. 6; p. 487
• Main Authors: Li, Chun-Yan, Zhao, Hua-Xin, Zhang, Xi, Chu, Li, Fang, Jue-Min, Han, Hui, Liu, Xi, Xu, Qing
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2013
• Subjects: Caffeic Acids - pharmacology; Cell Line, Tumor; Humans; Interleukin-6 - metabolism; Interleukin-6 - pharmacology; Male; NF-kappa B - antagonists & inhibitors; Phenylethyl Alcohol - analogs & derivatives; Phenylethyl Alcohol - pharmacology; Prostatic Neoplasms - metabolism; Signal Transduction - drug effects; STAT3 Transcription Factor - metabolism; Tumor Necrosis Factor-alpha - pharmacology
• ISSN: 1009-3591

To observe the changes in the expressions of STAT3 and NF-KB in PC-3 cells after IL-6 stimulation and to verify the effects of the NF-KB inhibitor caffeic acid phenethyl ester (CAPE) on the expressions of p-STAT3 and IL-6 in the PC-3 prostate cancer cell line. PC-3 prostate cancer cells were treated with IL-6 at 20 ng/ml for 5, 10, 20, 30 and 45 min. The protein and mRNA expressions of STAT3 and NF-kappaB were measured by Western blot and real time PCR, respectively, and the cell cycle was detected by flow cytometry. The PC-3 cells were exposed to TNF-alpha or TNF-alpha + CAPE, followed by determination of the IL-6 expression in the supernatant of the cells by ELISA and the expression of p-STAT3 by Western blot. After IL-6 stimulation, both the expression of p-STAT3 protein and the proliferation index of the PC-3 cells were significantly increased, and so were the expressions of IL-6 and p-STAT3 protein in the supernatant after TNF-alpha treatment (P < 0.05). TNF-alpha + CAPE induced statistically lower expre