GeneCalling: transcript profiling coupled to a gene database query

We describe here the GeneCalling method for the discovery of differentially expressed genes, both known and novel, from any species and with useful sequence information to determine the potential function of novel genes captured. The method relies on transcript visualization coupled to a database qu...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 317; p. 75
Main Author Shimkets, Richard A
Format Journal Article
LanguageEnglish
Published United States 2006
Subjects
Animals
Biotin - chemistry
Computational Biology - methods
Databases, Genetic
Disease Models, Animal
DNA, Complementary - metabolism
Gene Expression Profiling - methods
Gene Expression Regulation
Genetic Techniques
Humans
Neoplasms - genetics
Neoplasms - metabolism
Oligonucleotide Array Sequence Analysis
Oligonucleotides - chemistry
RNA - chemistry
RNA, Messenger - metabolism
Transcription, Genetic
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Summary:We describe here the GeneCalling method for the discovery of differentially expressed genes, both known and novel, from any species and with useful sequence information to determine the potential function of novel genes captured. The method relies on transcript visualization coupled to a database query to rapidly and quantitatively identify differentially expressed transcripts. The method has been applied to a wide variety of disease models in a wide variety of species, addressing problems as diverse as identifying novel human cancer gene targets, understanding how drugs and diet affect animal models of disease, and understanding the basis of trait differences in related strains of corn.
ISSN:1064-3745