The effects of monocyte-conditioned medium and interleukin 1 on the synthesis of collagenous and non-collagenous proteins by mouse bone and human bone cells in vitro

• Published in: Biochimica et biophysica acta, Protein structure and molecular enzymology Vol. 801; no. 1; pp. 58 - 65
• Main Authors: Beresford, J N, Gallagher, JA, Gowen, M, Couch, M, Poser, J, Wood, D D, Russell, RGG
• Format: Journal Article
• Language: English
• Published: 01.01.1984
• ISSN: 0167-4838

Cultured adherent human mononuclear cells produce factor(s) which stimulate the release of calcium from new-born mouse calvaria in organ culture. This stimulation of bone resorption is accompanied by an inhibition of the incorporation of ( super(3)H)proline into collagen which is dependent of increased prostaglandin production by the bone. When human osteoblast-like cells are treated with conditioned medium from human molecular cells, collagen accounts for a decreased proportion of the protein synthesised. In human osteoblast-like cell cultures, partially purified human interleukin 1 also inhibits the production of the bone-specific protein osteocalcin in a dose-dependent fashion.