Serum interleukin-1 receptor antagonist is an early indicator of colitis onset in Galphai2-deficient mice

• Published in: World journal of gastroenterology : WJG Vol. 12; no. 4; pp. 621 - 624
• Main Authors: Hultgren, O H, Berglund, M, Bjursten, M, Hultgren Hörnquist, E
• Format: Journal Article
• Language: English
• Published: United States 28.01.2006
• Subjects: Animals; Biological Markers; Biomarkers; blood; Colitis; Colitis - blood; Colitis - diagnosis; deficiency; diagnosis; genetics; Gi2; GTP-Binding Protein alpha Subunit; GTP-Binding Protein alpha Subunit, Gi2 - deficiency; GTP-Binding Protein alpha Subunit, Gi2 - genetics; GTP-Binding Protein alpha Subunit, Gi2 - physiology; Inbred C57BL; Inflammatory Bowel Diseases; Inflammatory Bowel Diseases - diagnosis; Interleukin 1 Receptor Antagonist Protein; Interleukin-18; Interleukin-18 - blood; Male; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; Mice; Mice, Inbred C57BL; Organ Size; pathology; physiology; Sialoglycoproteins; Sialoglycoproteins - blood; Spleen; Spleen - pathology
• ISSN: 1007-9327

To study the serum concentration of IL-1beta, IL-1 receptor antagonist (IL-1Ra) and IL-18 in Galphai2-deficient mice at the age of 6 (healthy), 12 (pre-colitic) and 24 wk (colitic) and in healthy control mice. At the time of killing, serum samples were collected and IL-1beta, IL-1Ra and IL-18 levels were measured using enzyme-linked immunosorbent assays. Serum concentration of IL-1Ra was significantly increased in pre-colitic (median: 524 ng/L; P=0.02) and colitic (450 ng/L; P=0.01), but not in healthy (196 ng/L) Galphai2-deficient mice as compared with controls (217 ng/L). Serum concentrations of IL-1beta did not differ between Galphai2-deficient mice and their controls, irrespective of age, IL-18 was significantly increased in colitic, but not in pre-colitic mice compared with controls (510 ng/L vs 190 ng/L; P=0.05). The increased serum concentrations of IL-18 and IL-1Ra in established diseases are suggested as markers of ongoing colitis. Interestingly, the significantly increased serum concentration of IL-1Ra in pre-colitic mice is found to be an early marker of disease progression.