Knockout of the hprK gene in B. subtilis CcpA mutant and its influence on riboflavin fermentation

In Bacillus subtilis , raising the amount of carbon catabolite in vivo would lead to carbon catabolite repression (CCR) and restrain the absorption of glucose. By deleting CcpA the CCR effect could be relieved, but the absorption of glucose remains restrained. The phosphoenol-pyruvate-sugar phosphot...

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Bibliographic Details
Published inShengwu gongcheng xuebao Vol. 22; no. 4; p. 534
Main Authors Zhang, Fan, Song, Hui, Ban, Rui
Format Journal Article
LanguageChinese
Published China 01.07.2006
Subjects
Bacillus subtilis - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - physiology
Fermentation
Glucose - metabolism
Mutation
Phosphoenolpyruvate Sugar Phosphotransferase System - genetics
Phosphoenolpyruvate Sugar Phosphotransferase System - physiology
Riboflavin - metabolism
Sucrose - metabolism
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Summary:In Bacillus subtilis , raising the amount of carbon catabolite in vivo would lead to carbon catabolite repression (CCR) and restrain the absorption of glucose. By deleting CcpA the CCR effect could be relieved, but the absorption of glucose remains restrained. The phosphoenol-pyruvate-sugar phosphotransferase system (PTS) is the main glucose transportation system in B. subtilis. HPr protein together with HprK/P participate in the glucose transportation. The HPr protein is phosphorylated at His-15 forming HPr-His-15-P transferring phosphate group from HPr to E II . While HprK/P phosphorylate HPr at Ser-46 forming HPr-Ser-46-P. HPr-Ser-46-P cannot participate in the transportation of glucose. The Knockout of ccpA gene increases the amount of fructose 1,6-bisphosphate(FBP) in vivo. And FBP could activate HPr kinase. So when CcpA is deleted, most part of the HPr will be phosphorylated at Ser-46. Absorpton of glucose is blocked. In this study, by disruption of hprk gene, the obtained B. subtilisZHc/pMX45 reaches t
ISSN:1000-3061