Optimization of the conditions for RAPD-PCR of Candida spp. and Cryptococcus spp

In this study, we determined the optimal RAPD amplification conditions to obtain genetic molecular markers for the rapid and accurate identification of Cryptococcus spp. and Candida spp. The following parameters are modified: template DNA, DNA polymerase, magnesium cloride and primer concentration;...

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Published inRevista iberoamericana de micología Vol. 18; no. 2; pp. 65 - 69
Main Authors Sansinforiano, M E, Rabasco, A, Martínez-Trancón, M, Parejo, J C, Hermoso-de-Mendoza, M, Padilla, J A
Format Journal Article
LanguageEnglish
Spanish
Published Spain 01.06.2001
Subjects
Candida
Cryptococcus
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Summary:In this study, we determined the optimal RAPD amplification conditions to obtain genetic molecular markers for the rapid and accurate identification of Cryptococcus spp. and Candida spp. The following parameters are modified: template DNA, DNA polymerase, magnesium cloride and primer concentration; denaturation, annealing and extension time, temperature of annealing and thermal cycles. After the optimization, reliable and reproducible RAPD patterns are obtained.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:1130-1406