A Protease-Activated Ratiometric Fluorescent Probe for pH-Mapping of Malignant Tumor

A protease-activated ratiometric fluorescent probe based on fluorescence resonance energy transfer between a pH-sensitive fluorescent dye and biocompatible Fe3O4 nanocrystals was constructed. A peptide substrate of MMP-9 served as a linker between the particle quencher and the chromophore that was c...

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Bibliographic Details
Published inAdvances in Science and Technology Vol. 102; pp. 51 - 56
Main Authors Shangguan, Di Hua, Gao, Ming Yuan, Yang, Wen Shang, Gao, Zhen Yu, Hou, Yi, Liu, Chun Yan, Zhou, Jin, Sun, Xiao Yu
Format Journal Article
LanguageEnglish
Published Zurich Trans Tech Publications Ltd 31.10.2016
Subjects
Smart Probe
Fe3O4 Nanocrystals
pH Mapping
Ratiometric Fluorescence
Protease-Activated
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Summary:A protease-activated ratiometric fluorescent probe based on fluorescence resonance energy transfer between a pH-sensitive fluorescent dye and biocompatible Fe3O4 nanocrystals was constructed. A peptide substrate of MMP-9 served as a linker between the particle quencher and the chromophore that was covalently attached to the antitumor antibody. The optical response of the probe to activated MMP-9 and gastric cell line SGC7901 tumor cells was investigated, followed by in vivo tumor imaging. Based on the ratiometric pH response to the tumor microenvironment, the resulting probe was successfully used to image the pH of subcutaneous tumor xenografts.
Bibliography:Proceedings of the 7th Forum on New Materials, including the 5th International Conference “Smart and Multifunctional Materials, Structures and Systems” and the 11th International Conference “Medical Applications of New Biomaterials and Nanotechnology, June 5-9, 2016, Perugia, Italy
ISSN:1662-8969
1662-0356
DOI:10.4028/www.scientific.net/AST.102.51