A Protease-Activated Ratiometric Fluorescent Probe for pH-Mapping of Malignant Tumor
A protease-activated ratiometric fluorescent probe based on fluorescence resonance energy transfer between a pH-sensitive fluorescent dye and biocompatible Fe3O4 nanocrystals was constructed. A peptide substrate of MMP-9 served as a linker between the particle quencher and the chromophore that was c...
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Published in | Advances in Science and Technology Vol. 102; pp. 51 - 56 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Zurich
Trans Tech Publications Ltd
31.10.2016
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Subjects | |
Online Access | Get full text |
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Summary: | A protease-activated ratiometric fluorescent probe based on fluorescence resonance energy transfer between a pH-sensitive fluorescent dye and biocompatible Fe3O4 nanocrystals was constructed. A peptide substrate of MMP-9 served as a linker between the particle quencher and the chromophore that was covalently attached to the antitumor antibody. The optical response of the probe to activated MMP-9 and gastric cell line SGC7901 tumor cells was investigated, followed by in vivo tumor imaging. Based on the ratiometric pH response to the tumor microenvironment, the resulting probe was successfully used to image the pH of subcutaneous tumor xenografts. |
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Bibliography: | Proceedings of the 7th Forum on New Materials, including the 5th International Conference “Smart and Multifunctional Materials, Structures and Systems” and the 11th International Conference “Medical Applications of New Biomaterials and Nanotechnology, June 5-9, 2016, Perugia, Italy |
ISSN: | 1662-8969 1662-0356 |
DOI: | 10.4028/www.scientific.net/AST.102.51 |