Modulation of 12-0-tetradecanoylphorbol-13-acetate-induced epidermal ornithine decarboxylase activity by calcium and verapamil in mouse

Calcium has been suggested to be an intracellular second messenger for ornithine decarboxylase (ODC) induction caused by 12-O-tetradecanoylphorbol-13-acetate (TPA). In the present study, the effects of dietary calcium supplement and calcium and verapamil injections on TPA-induced ODC activity in ski...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 199; no. 2; pp. 582 - 586
Main Authors CHANDRADHAR DWIVEDI, BAER, R. K, JARVIS, D. M
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier 15.03.1994
Subjects
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Calcium, Dietary - pharmacology
Enzyme Induction - drug effects
Enzymes and enzyme inhibitors
Female
Fundamental and applied biological sciences. Psychology
Kinetics
Lyases
Mice
Mice, Inbred Strains
Ornithine Decarboxylase - biosynthesis
Skin - drug effects
Skin - enzymology
Species Specificity
Tetradecanoylphorbol Acetate - pharmacology
Verapamil - pharmacology
Carbon-carbon lyases
Vertebrata
Mammalia
Enzymatic activity
Calcium
Mouse
Enzyme
Carboxy-lyases
Rodentia
Lyases
Ornithine decarboxylase
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Summary:Calcium has been suggested to be an intracellular second messenger for ornithine decarboxylase (ODC) induction caused by 12-O-tetradecanoylphorbol-13-acetate (TPA). In the present study, the effects of dietary calcium supplement and calcium and verapamil injections on TPA-induced ODC activity in skin was investigated in CD-1 and SENCAR mouse. Dietary calcium supplement did not influence ODC induction caused by TPA. However, calcium injections enhanced the TPA-induced ODC activity in CD-1 and SENCAR mouse skin. Verapamil injections resulted in a significant decrease in TPA-induced ODC activity in CD-1 and SENCAR mice. These results suggest that calcium and calcium antagonists may play a role in skin tumor promotion caused by TPA.
ISSN:0006-291X
1090-2104