Arabidopsis Nucleolin Affects Plant Development and Patterning1[W][OA]

Nucleolin is a major nucleolar protein implicated in many aspects of ribosomal biogenesis, including early events such as processing of the large 35S preribosomal RNA. We found that the Arabidopsis (Arabidopsis thaliana) parallel1 (parl1) mutant, originally identified by its aberrant leaf venation,...

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Bibliographic Details
Published inPlant physiology (Bethesda) Vol. 144; no. 1; pp. 173 - 186
Main Authors Jalean Joyanne Petricka, Nelson, Timothy Mark
Format Journal Article
LanguageEnglish
Published Rockville American Society of Plant Biologists 01.05.2007
Subjects
Leaves
Mutants
Yeasts
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Summary:Nucleolin is a major nucleolar protein implicated in many aspects of ribosomal biogenesis, including early events such as processing of the large 35S preribosomal RNA. We found that the Arabidopsis (Arabidopsis thaliana) parallel1 (parl1) mutant, originally identified by its aberrant leaf venation, corresponds to the Arabidopsis nucleolin gene. parl1 mutants display parallel leaf venation, aberrant localization of the provascular marker Athb8:beta-glucuronidase, the auxin-sensitive reporter DR5:beta-glucuronidase, and auxin-dependent growth defects. PARL1 is highly similar to the yeast (Saccharomyces cerevisiae) nucleolin NUCLEAR SIGNAL RECOGNITION 1 (NSR1) multifunctional protein; the Arabidopsis PARL1 gene can rescue growth defects of yeast nsr1 null mutants. This suggests that PARL1 protein may have roles similar to those of the yeast nucleolin in nuclear signal recognition, ribosomal processing, and ribosomal subunit accumulation. Based on the range of auxin-related defects in parl1 mutants, we propose that auxin-dependent organ growth and patterning is highly sensitive to the efficiency of nucleolin-dependent ribosomal processing.
Bibliography:Corresponding author; e-mail timothy.nelson@yale.edu; fax 203–432–3854.
The online version of this article contains Web-only data.
This work was supported by the National Science Foundation (grant no. IBN–0416731 to T.M.N.).
www.plantphysiol.org/cgi/doi/10.1104/pp.106.093575
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Timothy Mark Nelson (timothy.nelson@yale.edu).
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ISSN:0032-0889
1532-2548
DOI:10.1104/pp.106.093575