The role of fatty acids in the regulation of brush-border Ca2+ transport

• Published in: Biochimica et biophysica acta Vol. 777; no. 2; pp. 261 - 266
• Main Authors: KREUTTER, D, LAFRENIERE, D. C, RASMUSSEN, H
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier 07.11.1984; North-Holland
• Subjects: Acyl Coenzyme A - pharmacology; Animals; Biological and medical sciences; Biological Transport - drug effects; Calcium - metabolism; Cell physiology; Chickens; Fatty Acids - metabolism; Fatty Acids - pharmacology; Fundamental and applied biological sciences. Psychology; Intestinal Mucosa - metabolism; Male; Membrane and intracellular transports; Membrane Lipids - metabolism; Microvilli - metabolism; Molecular and cellular biology; Vitamin D Deficiency - metabolism; Vertebrata; Brush border; Regulation(control); Calcium; Enzyme; Membrane transport; Gut; Vitamin; Chicken; Aves; Calcium Ions; Fatty acids
• ISSN: 0006-3002; 1878-2434

The role of the fatty-acid composition of the intestinal brush-border membrane in the control of transmembrane Ca2+ transport was examined by in vitro acylation of endogenous phospholipids. The initial rate of Ca2+ uptake into brush-border membrane vesicles was stimulated 1.6-fold by incubation with 50 microM linoleoyl CoA, which was the most effective CoA ester examined. Oleoyl CoA was also active, but stearoyl CoA, palmitoyl CoA and arachidonyl CoA displayed no activity. The effect of linoleoyl CoA was specific for Ca2+ transport; sodium-dependent phosphate uptake was slightly inhibited and the activity of alkaline phosphatase, a brush-border enzyme, was unaffected. Incubation of brush-border vesicles with either stimulatory (oleate) or nonstimulatory (stearate) CoA esters resulted in the incorporation of fatty acid into the four major phospholipid classes, suggesting a fatty-acid specificity of the Ca2+ transport phenomenon. These results are consistent with the hypothesis that fatty acids are important elements in the control of brush-border Ca2+ transport.