Dynamic Proteomic Analysis Reveals a Switch between Central Carbon Metabolism and Alcoholic Fermentation in Rice Filling Grains1[W][OA]
Accumulation of reserve materials in filling grains involves the coordination of different metabolic and cellular processes, and understanding the molecular mechanisms underlying the interconnections remains a major challenge for proteomics. Rice (Oryza sativa) is an excellent model for studying gra...
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Published in | Plant physiology (Bethesda) Vol. 148; no. 2; pp. 908 - 925 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Rockville
American Society of Plant Biologists
01.10.2008
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Online Access | Get full text |
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Summary: | Accumulation of reserve materials in filling grains involves the coordination of different metabolic and cellular processes, and understanding the molecular mechanisms underlying the interconnections remains a major challenge for proteomics. Rice (Oryza sativa) is an excellent model for studying grain filling because of its importance as a staple food and the available genome sequence database. Our observations showed that embryo differentiation and endosperm cellularization in developing rice seeds were completed approximately 6 d after flowering (DAF); thereafter, the immature seeds mainly underwent cell enlargement and reached the size of mature seeds at 12 DAF. Grain filling began at 6 DAF and lasted until 20 DAF. Dynamic proteomic analyses revealed 396 protein spots differentially expressed throughout eight sequential developmental stages from 6 to 20 DAF and determined 345 identities. These proteins were involved in different cellular and metabolic processes with a prominently functional skew toward metabolism (45%) and protein synthesis/destination (20%). Expression analyses of protein groups associated with different functional categories/subcategories showed that substantially up-regulated proteins were involved in starch synthesis and alcoholic fermentation, whereas the down-regulated proteins in the process were involved in central carbon metabolism and most of the other functional categories/subcategories such as cell growth/division, protein synthesis, proteolysis, and signal transduction. The coordinated changes were consistent with the transition from cell growth and differentiation to starch synthesis and clearly indicated that a switch from central carbon metabolism to alcoholic fermentation may be important for starch synthesis and accumulation in the developmental process. |
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Bibliography: | The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Tai Wang (twang@ibcas.ac.cn). The online version of this article contains Web-only data. www.plantphysiol.org/cgi/doi/10.1104/pp.108.125633 This work was supported by the Chinese Ministry of Sciences and Technology (grant no. 2006CB910105) and the Chinese Academy of Sciences. Open Access articles can be viewed online without a subscription. Corresponding author; e-mail twang@ibcas.ac.cn. |
ISSN: | 0032-0889 1532-2548 |
DOI: | 10.1104/pp.108.125633 |