Association of Tat protein and viral mRNA with nuclear matrix from HIV-1-infected H9 cells

• Published in: Biochimica et biophysica acta Vol. 1008; no. 2; pp. 208 - 212
• Main Authors: MÜLLER, W. E. G, WENGER, R, REUTER, P, RENNEISEN, K, SCHRÖDER, H. C
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier 07.07.1989; North-Holland
• Subjects: AIDS/HIV; Biological and medical sciences; Cell Nucleus - metabolism; Cells, Cultured; Chelating Agents - pharmacology; DNA Probes; Fluorescein-5-isothiocyanate; Fluoresceins; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; Gene Products, tat; HIV-1 - genetics; HIV-1 - metabolism; Metalloproteins - metabolism; Microscopy, Fluorescence; Molecular and cellular biology; Molecular genetics; Phenanthrolines - pharmacology; RNA, Messenger - metabolism; RNA, Viral - metabolism; tat Gene Products, Human Immunodeficiency Virus; Thiocyanates; Transcription Factors - metabolism; Transcription. Transcription factor. Splicing. Rna processing; Cell culture; Nucleoskeleton; Transcription; Antibody; Retroviridae; Molecular interaction; Posttranscriptional modification; Virus; Ripening; Transactivating protein; Transmission electron microscopy; Messenger RNA; Chelating agent; Immunoblotting assay; Human immunodeficiency virus; Immunofluorescence; Divalent cation; Nuclear protein
• ISSN: 0006-3002; 1878-2434

The transactivating protein from human immunodeficiency virus type 1 (HIV-1), Tat, was found to bind to the nuclear matrix from uninfected and HIV-1-infected H9 cells. Addition of the Zn2+, Cd2+ and Cu2+ chelator o-phenanthroline destroyed the matrix fibrils and the binding affinity of Tat to the matrix. A sequential treatment of the matrix, first with o-phenanthroline and then with ZnCl2, partially restored the fibrillar-like matrix structure. Infection of H9 cells with HIV-1 resulted in a displacement of cellular mRNA by viral mRNA from the nuclear matrix. Both the matrix-bound host cell and HIV-1 mRNA were found to dissociate from the matrix in the presence of o-phenanthroline. This could be prevented by coincubation with Zn2+ or Cu2+ (but not Mg2+), which stabilize the mRNA containing nuclear matrix structure.