The human keratinocyte two-dimensional gel protein database (update 1992): towards an integrated approach to the study of cell proliferation, differentiation and skin diseases

The master two-dimensional gel database of human keratinocytes currently lists 2980 cellular proteins (2098 isoelectric focusing, IEF; and 882 nonequilibrium pH gradient electrophoresis, NEPHGE) many of which correspond to posttranslational modifications. About 20% of all recorded proteins have been...

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Bibliographic Details
Published inElectrophoresis Vol. 13; no. 12; p. 893
Main Authors Celis, J E, Rasmussen, H H, Madsen, P, Leffers, H, Honoré, B, Dejgaard, K, Gesser, B, Olsen, E, Gromov, P, Hoffmann, H J
Format Journal Article
LanguageEnglish
Published Germany 01.12.1992
Subjects
Cell Differentiation - physiology
Cell Division - physiology
Databases, Factual
Electrophoresis, Gel, Two-Dimensional
Humans
Keratinocytes - chemistry
Keratinocytes - cytology
Proteins - analysis
Reference Values
Skin Diseases - metabolism
Skin Diseases - pathology
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Summary:The master two-dimensional gel database of human keratinocytes currently lists 2980 cellular proteins (2098 isoelectric focusing, IEF; and 882 nonequilibrium pH gradient electrophoresis, NEPHGE) many of which correspond to posttranslational modifications. About 20% of all recorded proteins have been identified (protein name, organelle components, etc.) and they are listed in alphabetical order together with their M(r), pI, cellular localization and credit to the investigator(s) that aided in the identification. Also, we have listed 145 microsequenced proteins that are recorded in this database. As an aid in localizing the polypeptides we have included blow-ups of the master images (IEF, NEPHGE) displaying all the protein numbers. In the long run, the master keratinocyte database is expected to link protein and DNA sequencing and mapping information (Human Genome Program) and to provide an integrated picture of the expression levels and properties of the thousands of proteins that orchestrate various keratinocyte functions both in health and disease.
ISSN:0173-0835