Application of filter paper method for collection of blood for MLPA test

The results of MLPA test using serum and filter paper eluate have been compared in this paper. Testing 64 patient samples at 1:32 dilution, 31 were negative by both serum and eluate, 20 were positive by both, six were positive only by serum and one was positive only eluate. In six other cases eluate...

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Bibliographic Details
Published inIndian journal of leprosy Vol. 65; no. 2; p. 207
Main Authors Bhatia, V N, Chakraborty, S, Mukherjee, B, Panda, S N, Chakraborty, T K
Format Journal Article
LanguageEnglish
Published India 01.04.1993
Subjects
Agglutination Tests - methods
Blood Specimen Collection - methods
Humans
Leprosy - blood
Mycobacterium leprae
Reagent Strips
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Summary:The results of MLPA test using serum and filter paper eluate have been compared in this paper. Testing 64 patient samples at 1:32 dilution, 31 were negative by both serum and eluate, 20 were positive by both, six were positive only by serum and one was positive only eluate. In six other cases eluate gave equivocal results while serum result was clearly positive. Some eluates negative at 1:32 dilution gave weak positive agglutination at 1:16 dilution.
ISSN:0254-9395