Rabbit M type phosphoglyceromutase: comparative effects of two thiol reagents, antibody reaction and hybridization studies

• Published in: Comparative biochemistry and physiology. B, Comparative biochemistry Vol. 89; no. 2; pp. 257 - 262
• Main Authors: PREHU, M.-O, PREHU, C, CALVIN, M.-C, ROSA, R
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Science 1988
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Chickens; Electrophoresis, Polyacrylamide Gel; Enzymes and enzyme inhibitors; Ethylmaleimide - pharmacology; Fundamental and applied biological sciences. Psychology; Humans; Immunodiffusion; Iodoacetamide - pharmacology; Iodoacetates - pharmacology; Isoenzymes - metabolism; Isomerases; Phosphoglycerate Mutase - immunology; Phosphoglycerate Mutase - metabolism; Phosphotransferases - metabolism; Rabbits; Species Specificity; Human; Enzyme; Isozyme; Red blood cell; Rabbit; Hybridization; Lagomorpha; Striated muscle; Immunological method; Vertebrata; Mammalia; Bisphosphoglyceromutase; Phosphoglyceromutase; Comparative study; Physicochemical properties
• ISSN: 0305-0491

1. Treatment of purified rabbit phosphoglyceromutase (M type) with N-ethylmaleimide or with iodoacetamide produces the concurrent loss of phosphoglyceromutase activity with its collateral glycerate-2,3-P2 phosphatase activity. 2. Differences are observed in the protective effect of glycerate-2,3-P2 and of glycolate-2-P against N-ethylmaleimide and iodoacetamide treatments. 3. Specific chicken antibodies obtained by injection of the purified rabbit M type phosphoglyceromutase do not cross-react with the B type but neutralize both rabbit and human M type phosphoglyceromutase. 4. Purified rabbit M type phosphoglyceromutase can hybridize in vitro with the purified human B type or with purified human glycerate-2,3-P2 synthase. 5. Its ability to hybridize with glycerate-2,3-P2 synthase is unchanged after iodoacetamide treatment.