Structural polypeptides of a canine parvovirus: study by immunoadsorption and sequential analysis of infected cells. Brief report

The sequential appearance of three polypeptides, molecular weights (MW) 67,000, 70,000, 85,000 in cells infected with canine parvovirus (CPV) was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The viral origin of three polypeptides was confirmed by immunoadsorption using a sp...

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Bibliographic Details
Published inArchives of virology Vol. 82; no. 3-4; p. 233
Main Authors Surleraux, M, Burtonboy, G
Format Journal Article
LanguageEnglish
Published Austria 1984
Subjects
Animals
Antigen-Antibody Complex
Cats
Cell Line
Dog Diseases - microbiology
Dogs
Electrophoresis, Polyacrylamide Gel
Immune Sera
Immunosorbent Techniques
Kidney
Molecular Weight
Parvoviridae - analysis
Parvoviridae Infections - microbiology
Parvoviridae Infections - veterinary
Peptides - isolation & purification
Viral Proteins - isolation & purification
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Summary:The sequential appearance of three polypeptides, molecular weights (MW) 67,000, 70,000, 85,000 in cells infected with canine parvovirus (CPV) was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The viral origin of three polypeptides was confirmed by immunoadsorption using a specific antiserum, and polypeptides of similar MW were also demonstrated in purified CPV virions. A further polypeptide of MW 50,000 was also present in both infected cell extracts and purified virus. However the viral origin for this polypeptide could not be confirmed by immunoadsorption.
ISSN:0304-8608