The role of nuclear factor-kappaB essential modulator (NEMO) in B cell development and survival

The transcription factor nuclear factor-kappaB (NF-kappaB) is essential for immune and inflammatory responses. NF-kappaB essential modulator (NEMO) is a scaffolding component of the IkappaB kinase complex required for NF-kappaB activation in vitro. Because NF-kappaB activation is involved in B cell...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 100; no. 3; pp. 1203 - 1208
Main Authors Kim, Shinyop, La Motte-Mohs, Ross N A, Rudolph, Dorothea, Zuniga-Pflucker, Juan Carlos, Mak, Tak W
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 04.02.2003
Subjects
Animals
Antigens, CD19 - biosynthesis
B-Lymphocytes - cytology
B-Lymphocytes - immunology
B7-1 Antigen - biosynthesis
Cell Differentiation
Cell Line
Cell Separation
Cell Survival
Cellular biology
Coculture Techniques
Embryo, Mammalian - cytology
Flow Cytometry
I-kappa B Kinase
Immunoglobulin M - immunology
Mice
Protein Serine-Threonine Kinases - physiology
Stem Cells - cytology
Time Factors
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Summary:The transcription factor nuclear factor-kappaB (NF-kappaB) is essential for immune and inflammatory responses. NF-kappaB essential modulator (NEMO) is a scaffolding component of the IkappaB kinase complex required for NF-kappaB activation in vitro. Because NF-kappaB activation is involved in B cell development and function, we set out to determine whether NEMO is required for these processes. NEMO(-/-) mice die very early during embryogenesis, and fetal livers from NEMO(-/-) embryos can not reconstitute either B or T lymphopoiesis in irradiated host mice. We therefore used NEMO(-/-) embryonic stem cells and the OP9 in vitro differentiation system to demonstrate that NEMO is not required for B cell development but plays an important role in B cell survival.
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ISSN:0027-8424
1091-6490