Biological imaging of HEK293 cells expressing PLCgamma1 using surface-enhanced Raman microscopy

Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nano...

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Published inAnalytical chemistry (Washington) Vol. 79; no. 3; pp. 916 - 922
Main Authors Lee, Sangyeop, Kim, Sungyong, Choo, Jaebum, Shin, Soon Young, Lee, Young Han, Choi, Ha Young, Ha, Seunghan, Kang, Kyungho, Oh, Chil Hwan
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 01.02.2007
Subjects
Analytical chemistry
Antibodies, Monoclonal
Cancer
Cell Line
Cellular biology
Cytological Techniques
Diagnostic Imaging - methods
Gene expression
Gold
Humans
Metal Nanoparticles
Microscopy
Neoplasms - pathology
Phospholipase C gamma - analysis
Spectrum Analysis, Raman - methods
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Summary:Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCgamma1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.
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content type line 23
ISSN:0003-2700
1520-6882