Characterization of oligoadenylate synthetase-1 expression in rat mammary gland and prostate: effects of 17beta-estradiol on the regulation of OAS1g in both tissues

OAS1 belongs to a protein family of interferon-induced enzymes characterized by their ability to catalyze the synthesis of 2'-5'-linked oligomers of adenosine from ATP (2-5A). 2-5A bind to the latent Ribonuclease L (RNase L), which subsequently dimerizes into the active form, acquiring the...

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Published inMolecular and cellular biochemistry Vol. 314; no. 1-2; p. 113
Main Authors Maia, C J B, Socorro, S, Schmitt, F, Santos, C R A
Format Journal Article
LanguageEnglish
Published Netherlands 01.07.2008
Subjects
2',5'-Oligoadenylate Synthetase - genetics
2',5'-Oligoadenylate Synthetase - metabolism
Animals
Cell Nucleus - metabolism
Estradiol - pharmacology
Female
Gene Expression Regulation, Enzymologic - drug effects
Isoenzymes - genetics
Isoenzymes - metabolism
Male
Mammary Glands, Animal - drug effects
Mammary Glands, Animal - enzymology
Mammary Glands, Animal - metabolism
Mammary Glands, Animal - physiology
Prostate - drug effects
Prostate - enzymology
Prostate - metabolism
Prostate - physiology
Rats
Signal Transduction - drug effects
Signal Transduction - genetics
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Summary:OAS1 belongs to a protein family of interferon-induced enzymes characterized by their ability to catalyze the synthesis of 2'-5'-linked oligomers of adenosine from ATP (2-5A). 2-5A bind to the latent Ribonuclease L (RNase L), which subsequently dimerizes into the active form, acquiring the capacity of cleaving cellular and viral mRNA. Several studies indicate that OAS1 is an important inducer of apoptosis in human cancer cells and that it may be regulated by 17beta-estradiol (E(2)). The aim of this study was to characterize OAS1 gene expression in rat mammary gland and prostate, and to analyze its regulation by E(2) in both tissues. It is demonstrated that OAS1g is the most abundant OAS1 gene expressed in both tissues, and that OAS1 protein is present in the nucleus of rat mammary gland and prostate epithelial cells. In addition, it is shown by Real Time PCR that OAS1g is up-regulated by E(2) in rat mammary gland, but down-regulated in prostate, suggesting that the OAS1g gene may be related to estrogen dependent pathways in rat mammary gland and prostate physiology.
ISSN:0300-8177
DOI:10.1007/s11010-008-9771-z