Fully Automated Production and Characterization of 64Cu and Proof‐of‐Principle Small‐Animal PET Imaging Using 64Cu‐Labelled CA XII Targeting 6A10 Fab

64Cu is a cyclotron‐produced radionuclide which offers, thanks to its characteristic decay scheme, the possibility of combining positron emission tomography (PET) investigations with radiotherapy. We evaluated the Alceo system from Comecer SpA to automatically produce 64Cu for radiolabelling purpose...

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Bibliographic Details
Published inChemMedChem Vol. 13; no. 12; pp. 1230 - 1237
Main Authors Fiedler, Luise, Kellner, Markus, Oos, Rosel, Böning, Guido, Ziegler, Sibylle, Bartenstein, Peter, Zeidler, Reinhard, Gildehaus, Franz Josef, Lindner, Simon
Format Journal Article
LanguageEnglish
Published Weinheim Wiley Subscription Services, Inc 20.06.2018
Subjects
64Cu
6A10 Fab
Carbonic anhydrase
Carbonic anhydrases
Comecer Alceo
Cyclotrons
Diagnostic software
Diagnostic systems
Emission analysis
Fab
Glioma
In vivo methods and tests
Medical imaging
Muscles
Positron emission
Positron emission tomography
Radiation
Radiation protection
Radiation therapy
Radioisotopes
Radiolabelling
solid targets
Tomography
Tumors
Xenografts
Xenotransplantation
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Summary:64Cu is a cyclotron‐produced radionuclide which offers, thanks to its characteristic decay scheme, the possibility of combining positron emission tomography (PET) investigations with radiotherapy. We evaluated the Alceo system from Comecer SpA to automatically produce 64Cu for radiolabelling purposes. We established a 64Cu production routine with high yields and radionuclide purity in combination with excellent operator radiation protection. The carbonic anhydrase XII targeting 6A10 antibody Fab fragment was successfully radiolabelled with the produced 64Cu, and proof‐of‐principle small‐animal PET experiments on mice bearing glioma xenografts were performed. We obtained a high tumor‐to‐contralateral muscle ratio, which encourages further in vivo investigations of the radioconjugate regarding a possible application in diagnostic tumor imaging. Ideal decay: An automated 64Cu production system using the first‐generation Alceo setup was established and evaluated. Volume, pH, radionuclide purity, non‐radioactive metal impurities, and molar activity of the product were determined. The carbonic anhydrase XII targeting 6A10 Fab fragment was radiolabelled with the produced 64Cu, and proof‐of‐principle small‐animal PET imaging was performed.
ISSN:1860-7179
1860-7187
DOI:10.1002/cmdc.201800130