METHOD DEVELOPMENT AND VALIDATION ON RP-HPLC FOR ASSAY OF BULK AND FORMULATION OF CARMUSTINE

The isocratic RP-HPLC was used for method development of carmustine and simultaneous estimation of carmustine by using 4.6 mm X 250mm, 5-μm Shim-Pack solar (C18) column. Mobile phase was used contained a combination of Acetonitrile, water & buffer at the ratio of 4:5:1 respectively,flow rate was...

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Published inNeuroQuantology Vol. 20; no. 11; p. 5552
Main Authors Mote, Ganesh D, Bhor, Vedant B, Mullani, Ashish K, Kharat, Shubhangi S, Honmane, Sandip M, Sutar, Guruprasad V, Sajne, Sachin J, Saralaya, Mahesh G, Shinde, Anuja S, Nargatti, Prakash I, Patil, Nikhil D, Mali, Ramling D
Format Journal Article
LanguageEnglish
Published Bornova Izmir NeuroQuantology 01.01.2022
Subjects
Acetonitrile
Chromatography
Multiple myeloma
Pharmaceutical sciences
Pharmacology
Pharmacy
Regression coefficients
Research methodology
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Summary:The isocratic RP-HPLC was used for method development of carmustine and simultaneous estimation of carmustine by using 4.6 mm X 250mm, 5-μm Shim-Pack solar (C18) column. Mobile phase was used contained a combination of Acetonitrile, water & buffer at the ratio of 4:5:1 respectively,flow rate was 1.0 ml min-1 with a total run time of 20 minutes, column temperature was 37℃,observation was carried out at 254 nm using photo diode-array detector. Retention time found to be 13.270 & 13.10 minutes for pure carmustine and carmustine nanosuspension respectively and area for pure carmustine drug and carmustine nanosuspension was found to be 287059 &287056 respectively.And linear regression coefficient was found to be 0. 9972.Assay method was established for quantitative estimation of Carmustinefrom bulk drug & formulation
ISSN:1303-5150
DOI:10.14704/NQ.2022.20.11.NQ66553